BRENDA - Enzyme Database show
show all sequences of 1.1.1.372

Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina

Liepins, J.; Kuorelahti, S.; Penttilae, M.; Richard, P.; FEBS J. 273, 4229-4235 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Trichoderma reesei
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
methylglyoxal
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
D-glyceraldehyde
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
diacetyl
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
L-glyceraldehyde
at pH 7.0 and 30C
Trichoderma reesei
2.4
-
Glyoxal
at pH 7.0 and 30C
Trichoderma reesei
5.8
-
dihydroxyacetone
at pH 7.0 and 30C
Trichoderma reesei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36232
-
x * 36232, calculated from amino acid sequence
Trichoderma reesei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADPH + H+
Trichoderma reesei
-
glycerol + NADP+
-
-
?
D-glyceraldehyde + NADPH + H+
Trichoderma reesei RUT C-30
-
glycerol + NADP+
-
-
?
L-glyceraldehyde + NADPH + H+
Trichoderma reesei
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
glycerol + NADP+
-
-
?
L-glyceraldehyde + NADPH + H+
Trichoderma reesei RUT C-30
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
glycerol + NADP+
-
-
?
additional information
Trichoderma reesei
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
?
-
-
-
additional information
Trichoderma reesei RUT C-30
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trichoderma reesei
Q0GYU5
synonym Trichoderma reesei
-
Trichoderma reesei RUT C-30
Q0GYU5
synonym Trichoderma reesei
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Trichoderma reesei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADPH + H+
-
673558
Trichoderma reesei
glycerol + NADP+
-
-
-
?
D-glyceraldehyde + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
glycerol + NADP+
-
-
-
?
diacetyl + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
dihydroxyacetone + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
glyoxal + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
glyoxal + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
?
-
-
-
?
L-glyceraldehyde + NADPH + H+
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
673558
Trichoderma reesei
glycerol + NADP+
-
-
-
?
L-glyceraldehyde + NADPH + H+
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
673558
Trichoderma reesei RUT C-30
glycerol + NADP+
-
-
-
?
methylglyoxal + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
methylglyoxal + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
?
-
-
-
?
additional information
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
673558
Trichoderma reesei
?
-
-
-
-
additional information
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
673558
Trichoderma reesei RUT C-30
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 36232, calculated from amino acid sequence
Trichoderma reesei
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
specific cofactor
Trichoderma reesei
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae
Trichoderma reesei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
specific cofactor
Trichoderma reesei
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
methylglyoxal
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
D-glyceraldehyde
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
diacetyl
at pH 7.0 and 30C
Trichoderma reesei
0.9
-
L-glyceraldehyde
at pH 7.0 and 30C
Trichoderma reesei
2.4
-
Glyoxal
at pH 7.0 and 30C
Trichoderma reesei
5.8
-
dihydroxyacetone
at pH 7.0 and 30C
Trichoderma reesei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36232
-
x * 36232, calculated from amino acid sequence
Trichoderma reesei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde + NADPH + H+
Trichoderma reesei
-
glycerol + NADP+
-
-
?
D-glyceraldehyde + NADPH + H+
Trichoderma reesei RUT C-30
-
glycerol + NADP+
-
-
?
L-glyceraldehyde + NADPH + H+
Trichoderma reesei
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
glycerol + NADP+
-
-
?
L-glyceraldehyde + NADPH + H+
Trichoderma reesei RUT C-30
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
glycerol + NADP+
-
-
?
additional information
Trichoderma reesei
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
?
-
-
-
additional information
Trichoderma reesei RUT C-30
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Trichoderma reesei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde + NADPH + H+
-
673558
Trichoderma reesei
glycerol + NADP+
-
-
-
?
D-glyceraldehyde + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
glycerol + NADP+
-
-
-
?
diacetyl + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
dihydroxyacetone + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
glyoxal + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
glyoxal + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
?
-
-
-
?
L-glyceraldehyde + NADPH + H+
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
673558
Trichoderma reesei
glycerol + NADP+
-
-
-
?
L-glyceraldehyde + NADPH + H+
the activity with L-glyceraldehyde is only slightly lower compared to D-glyceraldehyde
673558
Trichoderma reesei RUT C-30
glycerol + NADP+
-
-
-
?
methylglyoxal + NADPH + H+
-
673558
Trichoderma reesei
?
-
-
-
?
methylglyoxal + NADPH + H+
-
673558
Trichoderma reesei RUT C-30
?
-
-
-
?
additional information
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
673558
Trichoderma reesei
?
-
-
-
-
additional information
no activity with NADH or glycerol. Very much reduced activity with dihydroxyacetone. No activity with C5 or C6 sugars (acetoin, D-ribose, D-xylose, and D-glucose)
673558
Trichoderma reesei RUT C-30
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36232, calculated from amino acid sequence
Trichoderma reesei
General Information
General Information
Commentary
Organism
metabolism
the enzyme is active in pathways for sugar acid catabolism such as D-galacturonate catabolism
Trichoderma reesei
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is active in pathways for sugar acid catabolism such as D-galacturonate catabolism
Trichoderma reesei
Other publictions for EC 1.1.1.372
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger
Fungal Genet. Biol.
45
1449-1457
2008
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1
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1
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1
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1
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1
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1
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673558
Liepins
Enzymes for the NADPH-dependen ...
Trichoderma reesei, Trichoderma reesei RUT C-30
FEBS J.
273
4229-4235
2006
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1
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6
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1
6
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3
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1
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12
1
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1
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1
1
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6
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1
6
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1
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12
1
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1
1
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