Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
apoenzyme, sitting drop vapor diffusion method, using 0.1 M CHES pH 9.5, 30% (w/v) PEG 400. Enzyme in complex with NAD+, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 5.5, 0.2 M MgCl2. Enzyme in complex with NAD+ and oxaloacetate, sitting drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glyoxysome | - |
Saccharomyces cerevisiae | 9514 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | Saccharomyces cerevisiae | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ Sepharose column chromatography, and Superdex 75 gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Saccharomyces cerevisiae | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37200, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Mdh3 | isoform | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | isoform MDH3 is an essential component of the gluconeogenesis pathway that generates glucose from noncarbohydrate carbon substrates and is involved in the reoxidation of NADH produced by fatty-acid beta-oxidation in glyoxysomes | Saccharomyces cerevisiae |