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Literature summary for 1.1.1.37 extracted from

  • Moriyama, S.; Nishio, K.; Mizushima, T.
    Structure of glyoxysomal malate dehydrogenase (MDH3) from Saccharomyces cerevisiae (2018), Acta Crystallogr. Sect. F, 74, 617-624 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, sitting drop vapor diffusion method, using 0.1 M CHES pH 9.5, 30% (w/v) PEG 400. Enzyme in complex with NAD+, sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M bis-Tris pH 5.5, 0.2 M MgCl2. Enzyme in complex with NAD+ and oxaloacetate, sitting drop vapor diffusion method, using 20% (w/v) PEG 10000, 0.1 M HEPES pH 7.5 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
glyoxysome
-
Saccharomyces cerevisiae 9514
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADH + H+ Saccharomyces cerevisiae
-
(S)-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ Sepharose column chromatography, and Superdex 75 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Saccharomyces cerevisiae (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 37200, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Mdh3 isoform Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism isoform MDH3 is an essential component of the gluconeogenesis pathway that generates glucose from noncarbohydrate carbon substrates and is involved in the reoxidation of NADH produced by fatty-acid beta-oxidation in glyoxysomes Saccharomyces cerevisiae