Cloned (Comment) | Organism |
---|---|
cMDH, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, expression of N-terminal His-tagged cMDH in Escherichia coli strain BL21 (DE3) pLysS | Taenia solium |
expression in Escherichia coli | Taenia solium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: praziquantel and albendazole | Taenia solium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Taenia solium | |
0.042 | - |
NAD+ | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
0.044 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication | Taenia solium | |
0.044 | - |
oxaloacetate | pH 8.0, 22°C, recombinant enzyme | Taenia solium | |
0.1 | - |
(S)-malate | pH 8.0, temperature not specified in the publication | Taenia solium | |
0.1 | - |
(S)-malate | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
0.167 | - |
NADH | pH 8.0, temperature not specified in the publication | Taenia solium | |
0.167 | - |
NADH | pH 8.0, 22°C, recombinant enzyme | Taenia solium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Taenia solium | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE | Taenia solium |
36000 | - |
x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein | Taenia solium |
36517 | - |
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE | Taenia solium |
36517 | - |
x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein | Taenia solium |
37340 | - |
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE | Taenia solium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Taenia solium | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Taenia solium | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taenia solium | F1C7I4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal His-tagged cMDH from Escherichia coli strain BL21(DE3) pLysS inclusion bodies by affinity chromatography to over 95% purity | Taenia solium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
409 | - |
pH 8.0, temperature not specified in the publication | Taenia solium |
409 | - |
purified recombinant enzyme, oxaloacetate reduction, pH 8.0, 22°C | Taenia solium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Taenia solium | oxaloacetate + NADH + H+ | - |
r | |
additional information | no substrate: lactate | Taenia solium | ? | - |
? | |
oxaloacetate + NADH + H+ | - |
Taenia solium | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE | Taenia solium |
? | x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein | Taenia solium |
Synonyms | Comment | Organism |
---|---|---|
cMDH | - |
Taenia solium |
cytosolic malate dehydrogenase | - |
Taenia solium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Taenia solium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 40 | gradual decrease in activity above 40°C | Taenia solium |
5 | 60 | purified His-tagged cMDH, high stability within this range | Taenia solium |
20 | - |
stable for at least 30 min | Taenia solium |
37 | - |
stable for at least 30 min | Taenia solium |
50.5 | - |
Tm, melting temperature | Taenia solium |
78.5 | - |
purified His-tagged cMDH, high stability within this range | Taenia solium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
47 | - |
(S)-malate | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
47.4 | - |
(S)-malate | pH 8.0, temperature not specified in the publication | Taenia solium | |
385 | - |
NAD+ | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
385.6 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Taenia solium | |
665 | - |
oxaloacetate | pH 8.0, 22°C, recombinant enzyme | Taenia solium | |
665.3 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication | Taenia solium | |
962 | - |
NADH | pH 8.0, 22°C, recombinant enzyme | Taenia solium | |
962.7 | - |
NADH | pH 8.0, temperature not specified in the publication | Taenia solium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
oxaloacetate reduction | Taenia solium |
8 | - |
- |
Taenia solium |
9.6 | - |
malate oxidation | Taenia solium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Taenia solium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | about 30% of the activity with NADH | Taenia solium | |
additional information | no activity with NADPH | Taenia solium | |
NAD+ | - |
Taenia solium | |
NADH | - |
Taenia solium | |
NADH | specific for NADH | Taenia solium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Taenia solium | isoelectrofocusing | - |
8.1 |
Taenia solium | calculated and isoelectric focusing | - |
8.1 |
Taenia solium | sequence calculation | - |
8.7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
474 | - |
(S)-malate | pH 8.0, temperature not specified in the publication | Taenia solium | |
474 | - |
(S)-malate | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
1510 | - |
oxaloacetate | pH 8.0, temperature not specified in the publication | Taenia solium | |
1880 | - |
NAD+ | pH 8.0, temperature not specified in the publication | Taenia solium | |
1880 | - |
NAD+ | pH 9.6, 22°C, recombinant enzyme | Taenia solium | |
5760 | - |
NADH | pH 8.0, temperature not specified in the publication | Taenia solium | |
5760 | - |
NADH | pH 8.0, 22°C, recombinant enzyme | Taenia solium | |
15100 | - |
oxaloacetate | pH 8.0, 22°C, recombinant enzyme | Taenia solium |