Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of Mg2+ to homogeneous enzyme stimulates protein aggregation and formation of enzyme tetramers | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
subunit, SDS-PAGE | Cereibacter sphaeroides |
75000 | - |
dimeric isoform, gel filtration | Cereibacter sphaeroides |
148000 | - |
tetrameric isoform, gel filtration | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cereibacter sphaeroides | the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway | ? | - |
? | |
additional information | Cereibacter sphaeroides 2R | the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Cereibacter sphaeroides 2R | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sephadex G25 gel filtration, and Sephadex G200 gel filtration | Cereibacter sphaeroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.246 | - |
crude homogenate, in 50 mM Tris-HCl, pH 8.0, at 25°C | Cereibacter sphaeroides |
11 | - |
tetrameric enzyme form, after 30.6fold purification, in 50 mM Tris-HCl, pH 8.0, at 25°C | Cereibacter sphaeroides |
14.7 | - |
dimeric enzyme form, after 40.1fold purification, in 50 mM Tris-HCl, pH 8.0, at 25°C | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Cereibacter sphaeroides | oxaloacetate + NADH + H+ | - |
? | |
(S)-malate + NAD+ | - |
Cereibacter sphaeroides 2R | oxaloacetate + NADH + H+ | - |
? | |
additional information | the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway | Cereibacter sphaeroides | ? | - |
? | |
additional information | the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway | Cereibacter sphaeroides 2R | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | increasing the pH from 7.0 to 8.5 causes association of dimers with formation of tetramers, while lowering of pH to 6.0 is accompanied by dissociation of enzyme dimers with formation of MDH monomers | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cereibacter sphaeroides |