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Literature summary for 1.1.1.37 extracted from

  • Eprintsev, A.T.; Klimova, M.A.; Shikhalieva, K.D.; Fedorin, D.N.; Dzhaber, M.T.; Kompantseva, E.I.
    Features of structural organization and expression regulation of malate dehydrogenase isoforms from Rhodobacter sphaeroides strain 2R (2009), Biochemistry, 74, 793-799.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of Mg2+ to homogeneous enzyme stimulates protein aggregation and formation of enzyme tetramers Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
subunit, SDS-PAGE Cereibacter sphaeroides
75000
-
dimeric isoform, gel filtration Cereibacter sphaeroides
148000
-
tetrameric isoform, gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cereibacter sphaeroides the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway ?
-
?
additional information Cereibacter sphaeroides 2R the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway ?
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides 2R
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sephadex G25 gel filtration, and Sephadex G200 gel filtration Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.246
-
crude homogenate, in 50 mM Tris-HCl, pH 8.0, at 25°C Cereibacter sphaeroides
11
-
tetrameric enzyme form, after 30.6fold purification, in 50 mM Tris-HCl, pH 8.0, at 25°C Cereibacter sphaeroides
14.7
-
dimeric enzyme form, after 40.1fold purification, in 50 mM Tris-HCl, pH 8.0, at 25°C Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Cereibacter sphaeroides oxaloacetate + NADH + H+
-
?
(S)-malate + NAD+
-
Cereibacter sphaeroides 2R oxaloacetate + NADH + H+
-
?
additional information the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway Cereibacter sphaeroides ?
-
?
additional information the dimeric 74-kDa isozyme is involved in tricarboxylic acid cycle, while the tetrameric 148-kDa isozyme takes part in the citramalate pathway Cereibacter sphaeroides 2R ?
-
?

Subunits

Subunits Comment Organism
More increasing the pH from 7.0 to 8.5 causes association of dimers with formation of tetramers, while lowering of pH to 6.0 is accompanied by dissociation of enzyme dimers with formation of MDH monomers Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
MDH
-
Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cereibacter sphaeroides