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Literature summary for 1.1.1.37 extracted from

  • Yennaco, L.J.; Hu, Y.; Holden, J.F.
    Characterization of malate dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum (2007), Extremophiles, 11, 741-746.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mdh, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pyrobaculum islandicum
gene mdh, DNA and amino acid sequence determination and analysis, phylogenetic tree Pyrobaculum islandicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information activity kinetics of the native and recombinant proteins are identical Pyrobaculum islandicum
0.015
-
oxaloacetate pH 8.0, 80°C Pyrobaculum islandicum
0.015
-
oxaloacetate recombinant enzyme, pH 8.0, 80°C Pyrobaculum islandicum
0.028
-
NAD+ recombinant enzyme, pH 8.0, 80°C Pyrobaculum islandicum
0.086
-
NADH pH 8.0, 80°C Pyrobaculum islandicum
0.086
-
NADH recombinant enzyme, pH 8.0, 80°C Pyrobaculum islandicum
0.5
-
L-malate recombinant enzyme, pH 8.0, 80°C Pyrobaculum islandicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
4 * 33000, SDS-PAGE Pyrobaculum islandicum
35000
-
4 * 35000, native enzyme, SDS-PAGE Pyrobaculum islandicum
150000
-
gel filtration Pyrobaculum islandicum
150000
-
native enzyme, gel filtration Pyrobaculum islandicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD(P)+ Pyrobaculum islandicum
-
oxaloacetate + NAD(P)H
-
r
(S)-malate + NAD+ Pyrobaculum islandicum
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Pyrobaculum islandicum
-
-
-
Pyrobaculum islandicum
-
a hyperthermophilic archaeon, strain DSM 4184
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 103fold by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Pyrobaculum islandicum
native enzyme 103fold to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration, recombinant His6-tagged enzyme by nickel affinity chromatography from Escherichia coli strain BL21(DE3) Pyrobaculum islandicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7
-
purified recombinant enzyme, oxidation reaction with oxaloacetate and NADPH Pyrobaculum islandicum
28
-
purified recombinant enzyme, reduction reaction with L-malate and NAD+ Pyrobaculum islandicum
310
-
purified native enzyme, reduction reaction with oxaloacetate and NADH Pyrobaculum islandicum
470
-
purified native enzyme Pyrobaculum islandicum
470
-
purified recombinant enzyme, reduction reaction with oxaloacetate and NADH Pyrobaculum islandicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD(P)+
-
Pyrobaculum islandicum oxaloacetate + NAD(P)H
-
r
(S)-malate + NAD+
-
Pyrobaculum islandicum oxaloacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
More peptide mass fingerprinting, overview Pyrobaculum islandicum
tetramer 4 * 33000, SDS-PAGE Pyrobaculum islandicum
tetramer 4 * 35000, native enzyme, SDS-PAGE Pyrobaculum islandicum

Synonyms

Synonyms Comment Organism
malate dehydrogenase
-
Pyrobaculum islandicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Pyrobaculum islandicum
92
-
probably above Pyrobaculum islandicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 92
-
Pyrobaculum islandicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Pyrobaculum islandicum

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
Pyrobaculum islandicum
5.5 11 activity range Pyrobaculum islandicum

Cofactor

Cofactor Comment Organism Structure
additional information no activity with malate and NADP+ Pyrobaculum islandicum
NAD+ dependent on Pyrobaculum islandicum
NAD+ preferred cofactor Pyrobaculum islandicum
NADH dependent on Pyrobaculum islandicum
NADH preferred cofactor Pyrobaculum islandicum
NADP+ 10% of the activity with NAD+ Pyrobaculum islandicum
NADPH 10% of the activity with NADH Pyrobaculum islandicum
NADPH less than 10% of maximal activity with NADH Pyrobaculum islandicum