Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | oxidation of malate to oxaloacetate | Corynebacterium glutamicum | |
isocitrate | oxidation of malate to oxaloacetate | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | reduction of oxaloacetate to malate and oxidation of malate to oxaloacetate | Corynebacterium glutamicum | |
2-oxoglutarate | reduction of oxaloacetate to malate and oxidation of malate to oxaloacetate | Corynebacterium glutamicum | |
citrate | reduction of oxaloacetate to malate | Corynebacterium glutamicum | |
isocitrate | reduction of oxaloacetate to malate | Corynebacterium glutamicum | |
succinate | slight inhibition of reduction of oxaloacetate | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
oxaloacetate | pH 6.5, 20-23°C | Corynebacterium glutamicum | |
0.041 | - |
NADH | pH 8.0, 20-23°C | Corynebacterium glutamicum | |
0.057 | - |
oxaloacetate | pH 8.0, 20-23°C | Corynebacterium glutamicum | |
0.07 | - |
NADH | pH 6.5, 20-23°C | Corynebacterium glutamicum | |
0.1 | - |
NADH | pH 9.5, 20-23°C | Corynebacterium glutamicum | |
0.2 | - |
oxaloacetate | pH 9.5, 20-23°C | Corynebacterium glutamicum | |
0.25 | - |
NADPH | pH 6.5, 20-23°C | Corynebacterium glutamicum | |
0.4 | - |
NAD+ | pH 10.5, 20-23°C | Corynebacterium glutamicum | |
0.55 | - |
oxaloacetate | pH 6.5, 20.23°C | Corynebacterium glutamicum | |
0.8 | - |
L-malate | pH 10.5, 20-23°C | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Corynebacterium glutamicum |
130000 | - |
gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NN33 | ATCC 14067 | - |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
517 | - |
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Corynebacterium glutamicum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Corynebacterium glutamicum | oxaloacetate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 0.05 mM. Reduction of oxalacetate to L-malate with NADPH as coenzyme | Corynebacterium glutamicum |
8 | - |
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 0.1 mM | Corynebacterium glutamicum |
9 | - |
around, activity is to low to depict a pH curve | Corynebacterium glutamicum |
9.5 | - |
reduction of oxaloacetate to L-malate with NADH as coenzyme, at oxaloacetate concentration of 1.0 mM | Corynebacterium glutamicum |
10.5 | - |
oxidation of L-malate to oxalacetete with NAD+ as coenzyme | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Corynebacterium glutamicum | |
NADH | almost equally active both for NADH and NADPH on the bases of the turnover number values at pH 6.5, which is optimal for both coenzymes | Corynebacterium glutamicum | |
NADP+ | - |
Corynebacterium glutamicum | |
NADPH | almost equally active both for NADH and NADPH on the bases of the turnover number values at pH 6.5, which is optimal for both coenzymes | Corynebacterium glutamicum |