Cloned (Comment) | Organism |
---|---|
Escherichia coli malate dehydrogenase gene cloned into expression vector pASK40 by PCR | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
oxaloacetate | mutant L305C | Escherichia coli | |
0.049 | - |
oxaloacetate | wild-type | Escherichia coli | |
0.052 | - |
NADH | mutant N122C-L305C(diamide) | Escherichia coli | |
0.056 | - |
NADH | mutant N122C-L305C(DTT) | Escherichia coli | |
0.061 | - |
NADH | wild-type | Escherichia coli | |
0.087 | - |
NADH | mutant L305C | Escherichia coli | |
0.26 | - |
NAD+ | wild-type | Escherichia coli | |
0.34 | - |
oxaloacetate | mutant N122C-L305C(DTT) | Escherichia coli | |
0.38 | - |
NAD+ | mutant N122C-L305C(DTT) | Escherichia coli | |
0.5 | - |
NAD+ | mutant N122C-L305C(diamide) | Escherichia coli | |
0.58 | - |
oxaloacetate | mutant N122C-L305C(diamide) | Escherichia coli | |
2.6 | - |
L-malate | wild-type | Escherichia coli | |
5.3 | - |
L-malate | mutant N122C-L305C(DTT) | Escherichia coli | |
6.8 | - |
L-malate | mutant N122C-L305C(diamide) | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast | - |
Pisum sativum | 9507 | - |
chloroplast | - |
Zea mays | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Spinacia oleracea | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Pisum sativum | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Zea mays | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Synechocystis sp. | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Synechocystis sp. 6803 CF | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P61889 | - |
- |
Pisum sativum | - |
pea | - |
Spinacia oleracea | - |
spinach | - |
Sus scrofa | - |
pig | - |
Synechocystis sp. | - |
- |
- |
Synechocystis sp. 6803 CF | - |
- |
- |
Zea mays | - |
maize | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Pisum sativum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Synechocystis sp. | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Synechocystis sp. 6803 CF | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Pisum sativum | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Zea mays | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Synechocystis sp. | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Synechocystis sp. 6803 CF | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | - |
Sus scrofa | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Spinacia oleracea | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Pisum sativum | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Zea mays | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Synechocystis sp. | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Synechocystis sp. 6803 CF | L-malate + NAD+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
mutant N122C-L305C | Escherichia coli |