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Literature summary for 1.1.1.365 extracted from

  • Lim, M.; Jeong, B.; Jung, M.; Harn, C.
    Transgenic tomato plants expressing strawberry D-galacturonic acid reductase gene display enhanced tolerance to abiotic stresses (2016), Plant Biotechnol. Rep., 10, 105-116 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene GalUR, recombinant stable overexpression in Solanum lycopersicum cv. H15 leaves and fruits using Agrobacterium tumefaciens strain EHA 105 transfection method, the pNW2300 vector, and the CaMV35S promoter. The transgenic plants show increased enzyme content and activity compared to wild-type. Semi-quantitative RT-PCR expression analysis, increased ascorbic acid content is correlated with high level of transcript expression, that is 2-3fold increased compared to wild-type Fragaria x ananassa

Protein Variants

Protein Variants Comment Organism
additional information transgenic tomatoes with increased ascorbic acid contents due to overexpression of the enzyme are found to be more tolerant to abiotic stresses induced by viologen, NaCl, or mannitol than non-transformed plants. In leaf disc senescence assay, the tolerance of these transgenic plants is better than control plants because they retain higher chlorophyll contents. Under salt stress of less than 200 mM NaCl. These transgenic plants survive, while control plants are unable to survive such high salt stress. Ascorbic acid contents in the transgenic plants are inversely correlated with malondialdehyde contents, especially under salt stress conditions. Higher expression levels of antioxidant genes (APX and CAT) are also found in these transgenic plants compared to that in the control plants. No detectable difference in SOD expression is found between transgenic plants and control plants. Phenotype, detailed overview Fragaria x ananassa

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa O49133 Fragaria chiloensis x Fragaria virginiana
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Source Tissue

Source Tissue Comment Organism Textmining
fruit
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Fragaria x ananassa
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leaf
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Fragaria x ananassa
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Synonyms

Synonyms Comment Organism
GalUR
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Fragaria x ananassa