BRENDA - Enzyme Database show
show all sequences of 1.1.1.365

The D-galacturonic acid catabolic pathway in Botrytis cinerea

Zhang, L.; Thiewes, H.; van Kan, J.A.; Fungal Genet. Biol. 48, 990-997 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Botrytis cinerea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
D-glucuronate
isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
1
-
D-glucuronate
isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
1.4
-
D-galacturonate
isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
2.5
-
D-galacturonate
isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36900
-
x * 36900, isoform GAR1, calculated from amino acid sequence
Botrytis cinerea
49200
-
x * 49200, isoform GAR2, calculated from amino acid sequence
Botrytis cinerea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Botrytis cinerea
-
L-galactonate + NADP+
-
-
r
D-galacturonate + NADPH + H+
Botrytis cinerea B05.10
-
L-galactonate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Botrytis cinerea
-
-
-
Botrytis cinerea B05.10
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
715098
Botrytis cinerea
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
715098
Botrytis cinerea B05.10
L-galactonate + NADP+
-
-
-
r
D-glucuronate + NADPH + H+
-
715098
Botrytis cinerea
?
-
-
-
r
D-glucuronate + NADPH + H+
-
715098
Botrytis cinerea B05.10
?
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 36900, isoform GAR1, calculated from amino acid sequence; x * 49200, isoform GAR2, calculated from amino acid sequence
Botrytis cinerea
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Botrytis cinerea
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Botrytis cinerea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Botrytis cinerea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
D-glucuronate
isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
1
-
D-glucuronate
isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
1.4
-
D-galacturonate
isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
2.5
-
D-galacturonate
isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C
Botrytis cinerea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36900
-
x * 36900, isoform GAR1, calculated from amino acid sequence
Botrytis cinerea
49200
-
x * 49200, isoform GAR2, calculated from amino acid sequence
Botrytis cinerea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-galacturonate + NADPH + H+
Botrytis cinerea
-
L-galactonate + NADP+
-
-
r
D-galacturonate + NADPH + H+
Botrytis cinerea B05.10
-
L-galactonate + NADP+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-galacturonate + NADPH + H+
-
715098
Botrytis cinerea
L-galactonate + NADP+
-
-
-
r
D-galacturonate + NADPH + H+
-
715098
Botrytis cinerea B05.10
L-galactonate + NADP+
-
-
-
r
D-glucuronate + NADPH + H+
-
715098
Botrytis cinerea
?
-
-
-
r
D-glucuronate + NADPH + H+
-
715098
Botrytis cinerea B05.10
?
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36900, isoform GAR1, calculated from amino acid sequence; x * 49200, isoform GAR2, calculated from amino acid sequence
Botrytis cinerea
Expression
Organism
Commentary
Expression
Botrytis cinerea
isoform GAR1 is induced about 10fold, while isoform GAR2 is strongly induced in cultures with D-galacturonic acid, pectate, and pectin as carbon source at 3 h after transfer, compared to continuous growth in glucose-containing culture
up
Expression (protein specific)
Organism
Commentary
Expression
Botrytis cinerea
isoform GAR1 is induced about 10fold, while isoform GAR2 is strongly induced in cultures with D-galacturonic acid, pectate, and pectin as carbon source at 3 h after transfer, compared to continuous growth in glucose-containing culture
up
Other publictions for EC 1.1.1.365
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741550
Li
-
Expression profiles of the ge ...
Rosa roxburghii
Acta Physiol. Plant.
39
44
2017
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742938
Matsubara
Fermentative production of L- ...
Naganishia diffluens, Naganishia diffluens OPU-FC11
J. Biosci. Bioeng.
122
639-644
2016
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
743438
Lim
-
Transgenic tomato plants expr ...
Fragaria x ananassa
Plant Biotechnol. Rep.
10
105-116
2016
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742163
Amaya
Increased antioxidant capacit ...
Fragaria x ananassa
Biotechnol. J.
10
490-500
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743465
Cai
-
Ectopic expression of FaGalUR ...
Fragaria x ananassa
Plant Growth Regul.
76
187-197
2015
-
1
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
741576
Sloothaak
Overexpression of the Aspergi ...
Aspergillus niger, Aspergillus niger CBS 120.49
AMB Express
4
66
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
741553
Yang
-
Accumulation of vitamin C and ...
Fragaria x ananassa
Adv. Mater. Res.
749
277-282
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721358
Kuivanen
Engineering filamentous fungi ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei
Appl. Environ. Microbiol.
78
8676-8683
2012
-
-
-
-
-
-
-
-
-
-
-
3
-
9
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725116
Venkatesh
-
Chlorophyll a fluorescence tra ...
Fragaria x ananassa
Hortic. Environ. Biotechnol.
53
320-328
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715098
Zhang
The D-galacturonic acid catabo ...
Botrytis cinerea, Botrytis cinerea B05.10
Fungal Genet. Biol.
48
990-997
2011
-
-
1
-
-
-
-
4
-
-
2
2
-
14
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
2
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
725952
Hemavath
-
Biochemical analysis of enhanc ...
Fragaria x ananassa
Mol. Breed.
28
105-115
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723997
Mojzita
Metabolic engineering of funga ...
Aspergillus niger, Aspergillus niger ATCC 1015, Trichoderma reesei, Trichoderma reesei QM6a
Appl. Environ. Microbiol.
76
169-175
2010
-
-
-
-
-
-
-
-
-
-
-
4
-
14
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726236
Hemavath
Over-expression of strawberry ...
Fragaria x ananassa
Plant Sci.
177
659-667
2009
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722316
Martens-Uzunova
An evolutionary conserved D-ga ...
Aspergillus niger, Aspergillus niger CBS 120.49
Fungal Genet. Biol.
45
1449-1457
2008
-
-
1
-
-
-
-
4
-
-
1
2
-
7
-
-
-
-
-
1
6
-
12
1
-
-
-
8
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
4
-
-
1
2
-
-
-
-
-
1
6
-
12
1
-
-
-
8
-
-
-
-
-
-
-
-
4
4
724580
Ishikawa
Functional characterization of ...
Euglena gracilis, Euglena gracilis Z
Biosci. Biotechnol. Biochem.
70
2720-2726
2006
2
-
-
-
-
-
1
4
-
2
2
2
-
22
-
-
1
-
-
1
2
-
16
1
-
-
-
3
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
1
-
4
-
2
2
2
-
-
-
1
-
1
2
-
16
1
-
-
-
3
1
1
-
-
1
1
1
1
3
3
724270
Kuorelahti
Identification in the mold Hyp ...
Trichoderma reesei, Trichoderma reesei RUT C-30
Biochemistry
44
11234-11240
2005
-
-
1
-
-
-
-
6
-
-
1
-
-
12
-
-
1
-
-
1
-
-
10
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
-
-
1
-
-
-
-
1
-
1
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726022
Agius
Engineering increased vitamin ...
Fragaria x ananassa
Nat. Biotechnol.
21
177-181
2003
-
-
1
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-