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Literature summary for 1.1.1.363 extracted from
- Cloning, expression, and characterization of a psychrophilic glucose 6-phosphate dehydrogenase from Sphingomonas sp. PAMC 26621 (2019), Int. J. Mol. Sci., 20, 1362 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sphingomonas sp. PAMC 26621 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 57% loss of activity. 5 mM, 64.8% loss of activity | Sphingomonas sp. PAMC 26621 |  |
| Fe3+ | 1 mM, 47.3% loss of activity. 5 mM, 95.2% loss of activity | Sphingomonas sp. PAMC 26621 | |
| Ni2+ | 1 mM, 71.4% loss of activity. Complete loss of activity | Sphingomonas sp. PAMC 26621 | |
| Zn2+ | 1 mM, 57% loss of activity. 5 mM, 93% loss of activity | Sphingomonas sp. PAMC 26621 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
D-glucose 6-phosphate | 20°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 0.145 | - |
D-glucose 6-phosphate | 30°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 0.675 | - |
D-glucose 6-phosphate | 20°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 4.3 | - |
D-glucose 6-phosphate | 30°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, slight activiation | Sphingomonas sp. PAMC 26621 | |
| Mg2+ | 5 mM, slight activiation | Sphingomonas sp. PAMC 26621 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NAD+ | Sphingomonas sp. PAMC 26621 | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | Sphingomonas sp. PAMC 26621 | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas sp. PAMC 26621 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sphingomonas sp. PAMC 26621 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NAD+ | - |
Sphingomonas sp. PAMC 26621 | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
| D-glucose 6-phosphate + NADP+ | - |
Sphingomonas sp. PAMC 26621 | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PD | - |
Sphingomonas sp. PAMC 26621 |
| glucose 6-phosphate dehydrogenase | - |
Sphingomonas sp. PAMC 26621 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
NAD+-linked reaction | Sphingomonas sp. PAMC 26621 |
| 30 | - |
NADP+-linked reaction | Sphingomonas sp. PAMC 26621 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 20 | the secondary structure of the enzyme is maintained between 4-40°C, its activity and tertiary structure are better preserved between 4-20°C | Sphingomonas sp. PAMC 26621 |
| 60 | - |
20 min, complete loss of activity | Sphingomonas sp. PAMC 26621 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.9 | - |
D-glucose 6-phosphate | 20°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 46.4 | - |
D-glucose 6-phosphate | 30°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 116.9 | - |
D-glucose 6-phosphate | 20°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 134.7 | - |
D-glucose 6-phosphate | 30°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Sphingomonas sp. PAMC 26621 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sphingomonas sp. PAMC 26621 | |
| NADP+ | - |
Sphingomonas sp. PAMC 26621 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | crucial regulatory enzyme in the oxidative pentose phosphate pathway that provides reductive potential in the form of NADPH, as well as carbon skeletons for the synthesis of macromolecules | Sphingomonas sp. PAMC 26621 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.8 | - |
D-glucose 6-phosphate | 30°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 29.5 | - |
D-glucose 6-phosphate | 20°C, NAD+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 899.2 | - |
D-glucose 6-phosphate | 20°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
| 929 | - |
D-glucose 6-phosphate | 30°C, NADP+-linked reaction, pH not specified in the publication | Sphingomonas sp. PAMC 26621 | |
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