Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibits competitively with respect to thionicotinamide-NAD+ | Leuconostoc mesenteroides | |
D-glucose 6-phosphate | high concentrations inhibit the NADP+-linked reaction in the dual wavelength assay (a method employing a mixture of one coenzyme and the thionicotinamide analog of the other coenzyme). Such inhibition is not observed in conventional assays using either NADP+ or thionicotinamide-NADP+ | Leuconostoc mesenteroides | |
NADH | inhibits noncompetitively with respect to thionicotinamide-NAD+ | Leuconostoc mesenteroides | |
NADPH | inhibits competitively with respect to thionicotinamide-NADP+ | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
thionicotinamide-NADP+ | pH 7.8, 25°C | Leuconostoc mesenteroides | |
0.006 | - |
NADP+ | pH 7.8, 25°C | Leuconostoc mesenteroides | |
0.007 | - |
D-glucose 6-phosphate | pH 7.8, 25°C, cosubstrate: thionicotinamide-NAD+ | Leuconostoc mesenteroides | |
0.009 | - |
D-glucose 6-phosphate | pH 7.8, 25°C, cosubstrate: thionicotinamide-NADP+ | Leuconostoc mesenteroides | |
0.011 | - |
thionicotinamide-NAD+ | pH 7.8, 25°C | Leuconostoc mesenteroides | |
0.053 | - |
D-glucose 6-phosphate | pH 7.8, 25°C, cosubstrate: NAD+ | Leuconostoc mesenteroides | |
0.081 | - |
D-glucose 6-phosphate | pH 7.8, 25°C, cosubstrate: NADP+ | Leuconostoc mesenteroides | |
0.106 | - |
NAD+ | pH 7.8, 25°C | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
a method is described which enables one to assay simultaneously the NAD+- and NADP+-linked reactions of dehydrogenases which can utilize both coenzymes. The method is based on the fact that the thionicotinamide analogs of NADH and NADPH absorb light maximally at 400 nm, a wavelength sufficiently far removed from the absorbance maximum of NADH and NADPH to permit measurements of the simultaneous reduction of NAD+ (or NADP+) and the thionicotinamide analog of NADP+ (or NAD+). Application of the method to glucosed 6-phosphate dehydrogenase from Leuconostoc mesenteroides reveals differential effects of glucose 6-phosphate concentration on the NAD+- and NADP+-linked reactions catalyzed by this enzyme which can not be detected by conventional assay procedures and which may have regulatory significance | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | iso ordered bi bi reaction | Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | ordered bi bi reaction | Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + thionicotinamide-NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + thionicotinamide-NADH + H+ | - |
? | |
D-glucose 6-phosphate + thionicotinamide-NADP+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + thionicotinamide-NADPH + H+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc mesenteroides | |
NADP+ | - |
Leuconostoc mesenteroides |