Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by iodoacetate, iodoacetamide, and p-hydroxymercuribenzoate | Leuconostoc mesenteroides | |
NADH | product inhibition. With NAD+ as the varied substrate and glucose 6-phosphate, nonsaturating, linear noncompetitive inhibition is observed. When an experiment is performed using saturating glucose 6-phosphate, the inhibition again appears to be noncompetitive. When glucose 6-phosphate is the varied substrate inhibition by NADH appears to be noncompetitive whether NAD+ is nonsaturating or saturating | Leuconostoc mesenteroides | |
NADPH | NADPH is tested as an alternate product inhibitor for the NAD+-linked reaction. With glucose 6-phosphate saturating and NAD+ varied the inhibition is linear noncompetitive | Leuconostoc mesenteroides | |
pyridoxal 5'-phosphate | inhibition in phosphate buffers at pH 7.7 and pH 6.3. 0.1 mM Pyridoxal 5-phosphate produces 50% inhibition at pH 7.7. At the lower pH more pyridoxal 5-phosphate is required to give the same degree of inhibition. The enzyme must be incubated with pyridoxal 5-phosphate prior to assaying in order to achieve maximum inhibition. Pyridoxamine 5-phosphate and pyridoxal are only slightly inhibitory. When glucose 6-phosphate is varied at a constant, nearly saturating concentration of NAD+ pyridoxal 5-phosphate behaves like a competitive inhibitor. When NAD+ is varied and glucose 6-phosphate maintained at nearly saturating concentration, inhibition by pyridoxal 5-phosphate is noncompetitive | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the Km-value of NAD+ is insensitive to ionic strength over the range tested but that the Km for glucose 6-phosphate is affected. This effect is greatest at pH 9.0 | Leuconostoc mesenteroides | |
0.006 | - |
NADP+ | 25°C, pH 7.2, cosubstrate: D-glucose 6-phosphate | Leuconostoc mesenteroides | |
0.011 | - |
thionicotinamide-NAD+ | 25°C, pH 7.2, cosubstrate: D-glucose 6-phosphate | Leuconostoc mesenteroides | |
0.053 | - |
D-glucose 6-phosphate | 25°C, pH 7.8, cosubstrate: NAD+ | Leuconostoc mesenteroides | |
0.081 | - |
D-glucose 6-phosphate | 25°C, pH 7.2, cosubstrate: NADP+ | Leuconostoc mesenteroides | |
0.106 | - |
NAD+ | 25°C, pH 7.8, cosubstrate: D-glucose 6-phosphate | Leuconostoc mesenteroides | |
10 | - |
D-galactose 6-phosphate | 25°C, pH 7.8, cosubstrate: NAD+ | Leuconostoc mesenteroides | |
12 | - |
2-deoxy-D-glucose 6-phosphate | 25°C, pH 7.8, cosubstrate: NAD+ | Leuconostoc mesenteroides | |
50 | - |
D-glucose 6-sulfate | 25°C, pH 7.8, cosubstrate: NAD+ | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NAD+ | Leuconostoc mesenteroides | - |
6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | Leuconostoc mesenteroides | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose 6-phosphate + NAD+ | low activity | Leuconostoc mesenteroides | ? | - |
? | |
D-galactose 6-phosphate + NAD+ | low activity | Leuconostoc mesenteroides | ? | - |
? | |
D-glucose 6-phosphate + NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NAD+ | the mechanism with NAD+ is more complex compared to the reaction with D-glucose 6-phosphate and NADP+ | Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + NADP+ | ordered, sequential mechanism for this reaction in which NADP+ is bound first to the enzyme and NADPH released last | Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
D-glucose 6-phosphate + thionicotinamide-NAD+ | - |
Leuconostoc mesenteroides | 6-phospho-D-glucono-1,5-lactone + thionicotinamide-NADH + H+ | - |
? | |
D-glucose 6-sulfate + NAD+ | low activity | Leuconostoc mesenteroides | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the mechanism with NAD+ is more complex compared to the reaction with D-glucose 6-phosphate and NADP+ | Leuconostoc mesenteroides | |
NADP+ | ordered, sequential mechanism for this reaction in which NADP+ is bound first to the enzyme and NADPH released last | Leuconostoc mesenteroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
pyridoxal 5'-phosphate | 25°C, pH 7.7, competitive inhibition when glucose 6-phosphate is varied at a constant, nearly saturating concentration of NAD+ | Leuconostoc mesenteroides |