Literature summary for 1.1.1.36 extracted from

Ploux, O.; Masamune, S.; Walsh, C.T.
The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli (1988), Eur. J. Biochem., 174, 177-182.

Search for more literature for 1.1.1.36

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM155	Zoogloea ramigera

Inhibitors

Inhibitors	Comment	Organism	Structure
acetoacetyl-CoA	above 0.01 mM	Zoogloea ramigera
propionylacetyl-CoA	-	Zoogloea ramigera

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.002	-	acetoacetyl-CoA	-	Zoogloea ramigera
0.002	-	propionylacetyl-CoA	-	Zoogloea ramigera
0.01	-	butyrylacetyl-CoA	-	Zoogloea ramigera
0.02	0.021	NADPH	cosubstrate acetoacetyl-CoA	Zoogloea ramigera
0.026	-	D-3-hydroxybutyryl-CoA	-	Zoogloea ramigera
0.74	-	2-methylacetoacetyl-CoA	-	Zoogloea ramigera
0.99	-	Acetoacetyl-S-(D-pantetheine)11-pivalate	-	Zoogloea ramigera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Zoogloea ramigera	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	4 * 24000 SDS-PAGE	Zoogloea ramigera
25200	-	4 * 25200 gene sequence	Zoogloea ramigera
100000	-	gel filtration	Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetoacetyl-CoA + NADPH	Zoogloea ramigera	biosynthesis of poly(D-3-hydroxybutyrate)	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Zoogloea ramigera	-	gene cloned in Escherichia coli	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sepharose chromatography, Blue gel column	Zoogloea ramigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
608	-	-	Zoogloea ramigera

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-methylacetoacetyl-CoA + NADPH	-	Zoogloea ramigera	2-methyl-3-hydroxybutyryl-CoA + NADP+	-	?
acetoacetyl-CoA + NADPH	biosynthesis of poly(D-3-hydroxybutyrate)	Zoogloea ramigera	?	-	?
acetoacetyl-CoA + NADPH + H+	-	Zoogloea ramigera	(3R)-3-hydroxybutyryl-CoA + NADP+	-	r
acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH	-	Zoogloea ramigera	3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+	-	?
butyrylacetyl-CoA + NADPH	-	Zoogloea ramigera	3-hydroxyhexanoyl-CoA + NADP+	-	?
propionylacetyl-CoA + NADPH	-	Zoogloea ramigera	3-hydroxypentanoyl-CoA + NADP+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 25200 gene sequence	Zoogloea ramigera
tetramer	4 * 24000 SDS-PAGE	Zoogloea ramigera

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9	-	butyrylacetyl-CoA	-	Zoogloea ramigera
27	-	2-methylacetoacetyl-CoA	-	Zoogloea ramigera
90	-	D-3-hydroxybutyryl-CoA	-	Zoogloea ramigera
124	-	propionylacetyl-CoA	-	Zoogloea ramigera
267	-	Acetoacetyl-S-(D-pantetheine)11-pivalate	-	Zoogloea ramigera
300	-	acetoacetyl-CoA	-	Zoogloea ramigera

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	specific for	Zoogloea ramigera
NADPH	B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA	Zoogloea ramigera
NADPH	absolute specificity	Zoogloea ramigera

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Release 2023.1 (January 2023)