Literature summary for 1.1.1.36 extracted from

  • Ploux, O.; Masamune, S.; Walsh, C.T.
    The NADPH-linked acetoacetyl-CoA reductase from Zoogloea ramigera. Characterization and mechanistic studies of the cloned enzyme over-produced in Escherichia coli (1988), Eur. J. Biochem., 174, 177-182.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM155 Zoogloea ramigera

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA above 0.01 mM Zoogloea ramigera
propionylacetyl-CoA
-
Zoogloea ramigera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.002
-
propionylacetyl-CoA
-
Zoogloea ramigera
0.74
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
0.01
-
butyrylacetyl-CoA
-
Zoogloea ramigera
0.026
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
0.99
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
0.02 0.021 NADPH cosubstrate acetoacetyl-CoA Zoogloea ramigera

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Zoogloea ramigera
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Zoogloea ramigera
24000
-
4 * 24000 SDS-PAGE Zoogloea ramigera
25200
-
4 * 25200 gene sequence Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetoacetyl-CoA + NADPH Zoogloea ramigera biosynthesis of poly(D-3-hydroxybutyrate) ?
-
?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
gene cloned in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose chromatography, Blue gel column Zoogloea ramigera

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
608
-
-
Zoogloea ramigera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+
-
Zoogloea ramigera (3R)-3-hydroxybutyryl-CoA + NADP+
-
r
acetoacetyl-S-(D-pantetheine)11-pivalate + NADPH
-
Zoogloea ramigera 3-hydroxybutyryl-S-(D-pantetheine)11-pivalate + NADP+
-
?
propionylacetyl-CoA + NADPH
-
Zoogloea ramigera 3-hydroxypentanoyl-CoA + NADP+
-
?
butyrylacetyl-CoA + NADPH
-
Zoogloea ramigera 3-hydroxyhexanoyl-CoA + NADP+
-
?
2-methylacetoacetyl-CoA + NADPH
-
Zoogloea ramigera 2-methyl-3-hydroxybutyryl-CoA + NADP+
-
?
acetoacetyl-CoA + NADPH biosynthesis of poly(D-3-hydroxybutyrate) Zoogloea ramigera ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 25200 gene sequence Zoogloea ramigera
tetramer 4 * 24000 SDS-PAGE Zoogloea ramigera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
butyrylacetyl-CoA
-
Zoogloea ramigera
27
-
2-methylacetoacetyl-CoA
-
Zoogloea ramigera
267
-
Acetoacetyl-S-(D-pantetheine)11-pivalate
-
Zoogloea ramigera
90
-
D-3-hydroxybutyryl-CoA
-
Zoogloea ramigera
300
-
acetoacetyl-CoA
-
Zoogloea ramigera
124
-
propionylacetyl-CoA
-
Zoogloea ramigera

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Zoogloea ramigera
NADPH B-specific, i.e. transfer of pro-S-hydrogen from NADPH to acetoacetyl-CoA Zoogloea ramigera
NADPH absolute specificity Zoogloea ramigera