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show all sequences of 1.1.1.358

Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH

Qin, H.M.; Yamamura, A.; Miyakawa, T.; Kataoka, M.; Maruoka, S.; Ohtsuka, J.; Nagata, K.; Shimizu, S.; Tanokura, M.; Proteins 81, 2059-2063 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta(DE3) cells
Candida parapsilosis
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 25% (w/v) PEG3350, and 0.2 M NaCl
Candida parapsilosis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydropantolactone + NADPH + H+
Candida parapsilosis
-
(R)-pantolactone + NADP+
-
-
?
2-dehydropantolactone + NADPH + H+
Candida parapsilosis IFO 0708
-
(R)-pantolactone + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida parapsilosis
Q76L36
-
-
Candida parapsilosis IFO 0708
Q76L36
-
-
Purification (Commentary)
Commentary
Organism
Ni Sepharose column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration
Candida parapsilosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydropantolactone + NADPH + H+
-
726465
Candida parapsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
-
726465
Candida parapsilosis IFO 0708
(R)-pantolactone + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Candida parapsilosis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta(DE3) cells
Candida parapsilosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
dependent on
Candida parapsilosis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 25% (w/v) PEG3350, and 0.2 M NaCl
Candida parapsilosis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-dehydropantolactone + NADPH + H+
Candida parapsilosis
-
(R)-pantolactone + NADP+
-
-
?
2-dehydropantolactone + NADPH + H+
Candida parapsilosis IFO 0708
-
(R)-pantolactone + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni Sepharose column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration
Candida parapsilosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-dehydropantolactone + NADPH + H+
-
726465
Candida parapsilosis
(R)-pantolactone + NADP+
-
-
-
?
2-dehydropantolactone + NADPH + H+
-
726465
Candida parapsilosis IFO 0708
(R)-pantolactone + NADP+
-
-
-
?
Other publictions for EC 1.1.1.358
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724098
Qin
Structure of conjugated polyke ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Appl. Microbiol. Biotechnol.
98
243-249
2013
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-
1
1
11
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
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-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
11
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726465
Qin
Crystal structure of conjugate ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Proteins
81
2059-2063
2013
-
-
1
1
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723839
Yamamura
Expression, purification, crys ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Acta Crystallogr. Sect. F
65
1145-1148
2009
-
-
1
1
-
-
-
-
-
-
1
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654311
Kataoka
Gene cloning and overexpressio ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Appl. Microbiol. Biotechnol.
64
359-366
2004
-
-
1
-
-
-
-
-
-
-
4
4
-
8
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
4
4
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347724
Hata
Ketopantoyl-lactone reductase ...
Candida parapsilosis, Candida parapsilosis IFO 0708
Biochim. Biophys. Acta
990
175-181
1989
-
-
-
1
-
-
12
4
-
-
3
1
-
6
-
-
1
-
-
-
2
-
6
1
-
-
2
-
-
-
2
1
4
1
-
-
-
-
1
1
-
-
-
12
4
4
-
-
3
1
-
-
-
1
-
-
2
-
6
1
-
-
2
-
-
-
2
1
-
-
-
-
-
-