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Literature summary for 1.1.1.357 extracted from

  • Nahoum, V.; Gangloff, A.; Legrand, P.; Zhu, D.W.; Cantin, L.; Zhorov, B.S.; Luu-The, V.; Labrie, F.; Breton, R.; Lin, S.X.
    Structure of the human 3alpha-hydroxysteroid dehydrogenase type 3 in complex with testosterone and NADP at 1.25-A resolution (2001), J. Biol. Chem., 276, 42091-42098.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the human 3alpha-HSD type 3 in the ternary complex with testosterone and NADP+ determined by molecular replacement method using the program EPMR, at 1.25-A resolution, binding site analysis Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for the 17beta-oxidation of testosterone is 0.00067 mM, and Km-value for the 17beta-reduction of androstenedione is 0.00138 mM Homo sapiens
0.0011
-
dihydrotestosterone pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P52895
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrotestosterone + NAD(P)H + H+
-
Homo sapiens 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
?
additional information the enzyme also exhibits a weak 17beta-hydroxysteroid dehydrogenase activity transforming androstenedione into testosterone Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
3alpha-HSD3
-
Homo sapiens
AKR1C2
-
Homo sapiens
type 3 3alpha-hydroxysteroid dehydrogenase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information Kcat-value for the 17beta-oxidation of testosterone is 0.000118 1/sec, and Kcat-value for the 17beta-reduction of androstenedione is 0.00112 1/sec Homo sapiens
0.025
-
dihydrotestosterone pH 7.5, 37°C Homo sapiens

General Information

General Information Comment Organism
metabolism enzyme is involved in the inactivation of steroid hormones Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information Kcat/Km-value for the 17beta-oxidation of testosterone is 0.183 1/sec*mM, and Kcat/Km-value for the 17beta-reduction of androstenedione is 0.817 1/sec*mM Homo sapiens
22.7
-
dihydrotestosterone pH 7.5, 37°C Homo sapiens