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Literature summary for 1.1.1.354 extracted from

  • Noge, K.; Kato, M.; Mori, N.; Kataoka, M.; Tanaka, C.; Yamasue, Y.; Nishida, R.; Kuwahara, Y.
    Geraniol dehydrogenase, the key enzyme in biosynthesis of the alarm pheromone, from the astigmatid mite Carpoglyphus lactis (Acari: Carpoglyphidae) (2008), FEBS J., 275, 2807-2817.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
geraniol pH 8.5, 22┬░C Carpoglyphus lactis
0.0595
-
NAD+ pH 8.5, 22┬░C Carpoglyphus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40630
-
1 * 40630, calculated from sequence Carpoglyphus lactis
42800
-
1 * 42800, SDS-PAGE Carpoglyphus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesol + NAD+ Carpoglyphus lactis key enzyme in biosynthesis of the alarm pheromone neral (2E,6E)-farnesal + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Carpoglyphus lactis B2NI94
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Carpoglyphus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
31
-
pH 8.5, 22┬░C Carpoglyphus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesol + NAD+ key enzyme in biosynthesis of the alarm pheromone neral Carpoglyphus lactis (2E,6E)-farnesal + NADH + H+
-
?
(2E,6E)-farnesol + NAD+ 50% of the activity compared to geraniol Carpoglyphus lactis (2E,6E)-farnesal + NADH + H+
-
?
3-methyl-2-buten-1-ol + NAD+ 4.3% of the activity compared to geraniol Carpoglyphus lactis 3-methyl-2-butenal + NADH + H+
-
?
citronellol + NAD+ 4.3% of the activity compared to geraniol Carpoglyphus lactis ?
-
?
geraniol + NAD+ nerol, the geometrical isomer of geraniol is inactive. The enzyme cannot use NADP+ as cofactor Carpoglyphus lactis geranial + NADH + H+
-
?
additional information straight-chain alcohols such as 1-octanol, 2-buten-1-ol, 1-butanol and ethanol serve as poor substrates Carpoglyphus lactis ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40630, calculated from sequence Carpoglyphus lactis
monomer 1 * 42800, SDS-PAGE Carpoglyphus lactis

Synonyms

Synonyms Comment Organism
GeDH
-
Carpoglyphus lactis
NAD+-farnesol dehydrogenase
-
Carpoglyphus lactis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
-
Carpoglyphus lactis

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
5 60 5┬░C: about 50% of maximal activity, 60┬░C: about 50% of maximal activity Carpoglyphus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2996
-
geraniol pH 8.5, 22┬░C Carpoglyphus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Carpoglyphus lactis

pH Range

pH Minimum pH Maximum Comment Organism
8 10.5 pH 8.0: about 75% of maximal activity, pH 10.5: about 35% of maximal activity Carpoglyphus lactis

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme cannot use NADP+ as cofactor Carpoglyphus lactis

General Information

General Information Comment Organism
physiological function key enzyme in biosynthesis of the alarm pheromone neral Carpoglyphus lactis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
58400
-
geraniol pH 8.5, 22┬░C Carpoglyphus lactis