BRENDA - Enzyme Database show
show all sequences of 1.1.1.352

Purification and gene cloning of a dehydrogenase from Lactobacillus brevis that catalyzes a reaction involved in aflatoxin biosynthesis

Sakuno, E.; Kameyama, M.; Nakajima, H.; Yabe, K.; Biosci. Biotechnol. Biochem. 72, 724-734 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Lactobacillus brevis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0426
-
5'-hydroxyaverantin
in 30 mM potassium phosphate buffer (pH 7.5), at 37°C
Lactobacillus brevis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25873
-
3 * 25873, calculated from amino acid sequence
Lactobacillus brevis
25918
-
3 * 25918 MALDI TOF mass spectrometry
Lactobacillus brevis
33000
-
3 * 33000, SDS-PAGE
Lactobacillus brevis
100000
-
gel filtration
Lactobacillus brevis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-hydroxyaverantin + NADP+
Lactobacillus brevis
-
5'-oxoaverantin + NADPH + H+
-
-
ir
5'-hydroxyaverantin + NADP+
Lactobacillus brevis IFO 12005
-
5'-oxoaverantin + NADPH + H+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus brevis
-
-
-
Lactobacillus brevis IFO 12005
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration
Lactobacillus brevis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00613
-
cell extract, at pH 7.5 and 37°C
Lactobacillus brevis
0.552
-
after 90fold purification, at pH 7.5 and 37°C
Lactobacillus brevis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-hydroxyaverantin + NADP+
-
721863
Lactobacillus brevis
5'-oxoaverantin + NADPH + H+
-
-
-
ir
5'-hydroxyaverantin + NADP+
-
721863
Lactobacillus brevis IFO 12005
5'-oxoaverantin + NADPH + H+
-
-
-
ir
additional information
no activity with NAD+
721863
Lactobacillus brevis
?
-
-
-
-
additional information
no activity with NAD+
721863
Lactobacillus brevis IFO 12005
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 25873, calculated from amino acid sequence; 3 * 25918 MALDI TOF mass spectrometry; 3 * 33000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
55
-
Lactobacillus brevis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
no activity is detected above pH 9.0
Lactobacillus brevis
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Lactobacillus brevis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Lactobacillus brevis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
no activity with NAD+
Lactobacillus brevis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0426
-
5'-hydroxyaverantin
in 30 mM potassium phosphate buffer (pH 7.5), at 37°C
Lactobacillus brevis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25873
-
3 * 25873, calculated from amino acid sequence
Lactobacillus brevis
25918
-
3 * 25918 MALDI TOF mass spectrometry
Lactobacillus brevis
33000
-
3 * 33000, SDS-PAGE
Lactobacillus brevis
100000
-
gel filtration
Lactobacillus brevis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-hydroxyaverantin + NADP+
Lactobacillus brevis
-
5'-oxoaverantin + NADPH + H+
-
-
ir
5'-hydroxyaverantin + NADP+
Lactobacillus brevis IFO 12005
-
5'-oxoaverantin + NADPH + H+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration
Lactobacillus brevis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00613
-
cell extract, at pH 7.5 and 37°C
Lactobacillus brevis
0.552
-
after 90fold purification, at pH 7.5 and 37°C
Lactobacillus brevis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-hydroxyaverantin + NADP+
-
721863
Lactobacillus brevis
5'-oxoaverantin + NADPH + H+
-
-
-
ir
5'-hydroxyaverantin + NADP+
-
721863
Lactobacillus brevis IFO 12005
5'-oxoaverantin + NADPH + H+
-
-
-
ir
additional information
no activity with NAD+
721863
Lactobacillus brevis
?
-
-
-
-
additional information
no activity with NAD+
721863
Lactobacillus brevis IFO 12005
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 25873, calculated from amino acid sequence; 3 * 25918 MALDI TOF mass spectrometry; 3 * 33000, SDS-PAGE
Lactobacillus brevis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
55
-
Lactobacillus brevis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
no activity is detected above pH 9.0
Lactobacillus brevis
Other publictions for EC 1.1.1.352
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721863
Sakuno
Purification and gene cloning ...
Lactobacillus brevis, Lactobacillus brevis IFO 12005
Biosci. Biotechnol. Biochem.
72
724-734
2008
-
-
1
-
-
-
-
1
-
-
4
2
-
7
-
-
1
-
-
-
2
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
4
2
-
-
-
1
-
-
2
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
721331
Sakuno
Involvement of two cytosolic e ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Appl. Environ. Microbiol.
69
6418-6426
2003
-
-
-
-
-
-
-
1
1
-
3
2
-
10
-
-
1
-
-
1
2
-
2
1
1
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
3
2
-
-
-
1
-
1
2
-
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
721327
Chang
adhA in Aspergillus parasiticu ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 2043, Aspergillus parasiticus SU-1
Appl. Environ. Microbiol.
66
4715-4719
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723018
Sakuno
A new inhibitor of 5-hydroxyav ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
J. Nat. Prod.
63
1677-1678
2000
-
-
-
-
-
-
1
-
1
-
-
-
-
10
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-