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Literature summary for 1.1.1.352 extracted from

  • Sakuno, E.; Kameyama, M.; Nakajima, H.; Yabe, K.
    Purification and gene cloning of a dehydrogenase from Lactobacillus brevis that catalyzes a reaction involved in aflatoxin biosynthesis (2008), Biosci. Biotechnol. Biochem., 72, 724-734.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0426
-
5'-hydroxyaverantin in 30 mM potassium phosphate buffer (pH 7.5), at 37°C Levilactobacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Levilactobacillus brevis
33000
-
3 * 33000, SDS-PAGE Levilactobacillus brevis
25873
-
3 * 25873, calculated from amino acid sequence Levilactobacillus brevis
25918
-
3 * 25918 MALDI TOF mass spectrometry Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-hydroxyaverantin + NADP+ Levilactobacillus brevis
-
5'-oxoaverantin + NADPH + H+
-
ir
5'-hydroxyaverantin + NADP+ Levilactobacillus brevis IFO 12005
-
5'-oxoaverantin + NADPH + H+
-
ir

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis
-
-
-
Levilactobacillus brevis IFO 12005
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose CL-6B column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration Levilactobacillus brevis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00613
-
cell extract, at pH 7.5 and 37°C Levilactobacillus brevis
0.552
-
after 90fold purification, at pH 7.5 and 37°C Levilactobacillus brevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NAD+ Levilactobacillus brevis ?
-
?
additional information no activity with NAD+ Levilactobacillus brevis IFO 12005 ?
-
?
5'-hydroxyaverantin + NADP+
-
Levilactobacillus brevis 5'-oxoaverantin + NADPH + H+
-
ir
5'-hydroxyaverantin + NADP+
-
Levilactobacillus brevis IFO 12005 5'-oxoaverantin + NADPH + H+
-
ir

Subunits

Subunits Comment Organism
homotrimer 3 * 33000, SDS-PAGE Levilactobacillus brevis
homotrimer 3 * 25873, calculated from amino acid sequence Levilactobacillus brevis
homotrimer 3 * 25918 MALDI TOF mass spectrometry Levilactobacillus brevis

Synonyms

Synonyms Comment Organism
alcohol dehydrogenase
-
Levilactobacillus brevis
HAVN dehydrogenase
-
Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 55
-
Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 no activity is detected above pH 9.0 Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
NADP+ no activity with NAD+ Levilactobacillus brevis