BRENDA - Enzyme Database show
show all sequences of 1.1.1.352

Involvement of two cytosolic enzymes and a novel intermediate, 5-oxoaverantin, in the pathway from 5-hydroxyaverantin to averufin in aflatoxin biosynthesis

Sakuno, E.; Yabe, K.; Nakajima, H.; Appl. Environ. Microbiol. 69, 6418-6426 (2003)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
5'-hydroxyaverantin
in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Aspergillus parasiticus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Aspergillus parasiticus
29600
-
2 * 29600, calculated from amino acid sequence
Aspergillus parasiticus
60000
-
gel filtration
Aspergillus parasiticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-hydroxyaverantin + NAD+
Aspergillus parasiticus
-
5'-oxoaverantin + NADH + H+
-
-
?
5'-hydroxyaverantin + NAD+
Aspergillus parasiticus NIAH-26
-
5'-oxoaverantin + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus parasiticus
-
-
-
Aspergillus parasiticus NIAH-26
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DE 52 column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
Aspergillus parasiticus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000032
-
crude enzyme, in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
0.0222
-
after 694fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-hydroxyaverantin + NAD+
-
721331
Aspergillus parasiticus
5'-oxoaverantin + NADH + H+
-
-
-
?
5'-hydroxyaverantin + NAD+
-
721331
Aspergillus parasiticus NIAH-26
5'-oxoaverantin + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 28000, SDS-PAGE; 2 * 29600, calculated from amino acid sequence
Aspergillus parasiticus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
-
Aspergillus parasiticus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
40
-
Aspergillus parasiticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Aspergillus parasiticus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required for activity
Aspergillus parasiticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required for activity
Aspergillus parasiticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.083
-
5'-hydroxyaverantin
in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Aspergillus parasiticus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28000
-
2 * 28000, SDS-PAGE
Aspergillus parasiticus
29600
-
2 * 29600, calculated from amino acid sequence
Aspergillus parasiticus
60000
-
gel filtration
Aspergillus parasiticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5'-hydroxyaverantin + NAD+
Aspergillus parasiticus
-
5'-oxoaverantin + NADH + H+
-
-
?
5'-hydroxyaverantin + NAD+
Aspergillus parasiticus NIAH-26
-
5'-oxoaverantin + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DE 52 column chromatography, butyl Toyopearl column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
Aspergillus parasiticus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus parasiticus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.000032
-
crude enzyme, in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
0.0222
-
after 694fold purification, in 60 mM potassium phosphate buffer (pH 7.5), at 30C
Aspergillus parasiticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5'-hydroxyaverantin + NAD+
-
721331
Aspergillus parasiticus
5'-oxoaverantin + NADH + H+
-
-
-
?
5'-hydroxyaverantin + NAD+
-
721331
Aspergillus parasiticus NIAH-26
5'-oxoaverantin + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 28000, SDS-PAGE; 2 * 29600, calculated from amino acid sequence
Aspergillus parasiticus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
35
-
Aspergillus parasiticus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
40
-
Aspergillus parasiticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Aspergillus parasiticus
Other publictions for EC 1.1.1.352
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721863
Sakuno
Purification and gene cloning ...
Lactobacillus brevis, Lactobacillus brevis IFO 12005
Biosci. Biotechnol. Biochem.
72
724-734
2008
-
-
1
-
-
-
-
1
-
-
4
2
-
7
-
-
1
-
-
-
2
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
4
2
-
-
-
1
-
-
2
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
721331
Sakuno
Involvement of two cytosolic e ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Appl. Environ. Microbiol.
69
6418-6426
2003
-
-
-
-
-
-
-
1
1
-
3
2
-
10
-
-
1
-
-
1
2
-
2
1
1
1
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
3
2
-
-
-
1
-
1
2
-
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
721327
Chang
adhA in Aspergillus parasiticu ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 2043, Aspergillus parasiticus SU-1
Appl. Environ. Microbiol.
66
4715-4719
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723018
Sakuno
A new inhibitor of 5-hydroxyav ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
J. Nat. Prod.
63
1677-1678
2000
-
-
-
-
-
-
1
-
1
-
-
-
-
10
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-