Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme can be used as marker for early immunonological detection of infection by Leptospira in urine and of leptospirosis, a global zoonosis caused by pathogenic Leptospira | Leptospira interrogans serovar Copenhageni |
Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli | Leptospira interrogans serovar Copenhageni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Leptospira interrogans serovar Copenhageni | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47865 | - |
- |
Leptospira interrogans serovar Copenhageni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | Leptospira interrogans serovar Copenhageni | - |
3-oxoacyl-CoA + NADH + H+ | - |
? | |
(S)-3-hydroxyacyl-CoA + NAD+ | Leptospira interrogans serovar Copenhageni Fiocruz LV4231 | - |
3-oxoacyl-CoA + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans serovar Copenhageni | Q72M90 | - |
- |
Leptospira interrogans serovar Copenhageni Fiocruz LV4231 | Q72M90 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by Co2+ affinity chromatography | Leptospira interrogans serovar Copenhageni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme detection in urine of infected Mesocricetus auratus: leptospiral protein, 3-hydroxyacyl-CoA dehydrogenase (HADH), is found to be excreted in the urine of hamsters during the early phase of infection | Leptospira interrogans serovar Copenhageni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxyacyl-CoA + NAD+ | - |
Leptospira interrogans serovar Copenhageni | 3-oxoacyl-CoA + NADH + H+ | - |
? | |
(S)-3-hydroxyacyl-CoA + NAD+ | - |
Leptospira interrogans serovar Copenhageni Fiocruz LV4231 | 3-oxoacyl-CoA + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant His-tagged enzyme, SDS-PAGE, x * 47865, sequence calculation | Leptospira interrogans serovar Copenhageni |
? | x * 60000, native enzyme isolated from hamster urine, SDS-PAGE | Leptospira interrogans serovar Copenhageni |
Synonyms | Comment | Organism |
---|---|---|
HADH | - |
Leptospira interrogans serovar Copenhageni |
LIC13300 | - |
Leptospira interrogans serovar Copenhageni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leptospira interrogans serovar Copenhageni |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme specifically catalyzes the third step of beta oxidation | Leptospira interrogans serovar Copenhageni |
physiological function | the enzyme is classified as an oxidoreductase in fatty acid metabolic processes. It specifically catalyzes the third step of beta oxidation. Long-chain fatty acids are utilized by Leptospira as the sole carbon source and are metabolized by beta-oxidation. Therefore, a large amount of HADH may be produced intracellularly and released to get carbons and energy by oxidizing free fatty acid | Leptospira interrogans serovar Copenhageni |