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Literature summary for 1.1.1.35 extracted from

  • Taylor, R.C.; Brown, A.K.; Singh, A.; Bhatt, A.; Besra, G.S.
    Characterization of a beta-hydroxybutyryl-CoA dehydrogenase from Mycobacterium tuberculosis (2010), Microbiology, 156, 1975-1982.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 1 mM, about 50% inhibition Mycobacterium tuberculosis
EDTA about 35% inhibition at 1 mM, about 25% inhibition at 10 mM Mycobacterium tuberculosis
K+ about 30% inhibition at 1 mM, about 20% inhibition at 10 mM Mycobacterium tuberculosis
Ni2+ 1 mM, about 60% inhibition Mycobacterium tuberculosis
Zn2+ 1 mM, about 60% inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
29.5
-
NAD+ pH 9.5, 30°C Mycobacterium tuberculosis
43.5
-
(S)-3-hydroxybutyryl-CoA pH 9.5, 30°C Mycobacterium tuberculosis
50
-
NADH pH 7.0, 30°C Mycobacterium tuberculosis
65.6
-
3-acetoacetyl-CoA pH 7.0, 30°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances activity at 1 mM or 10 mM Mycobacterium tuberculosis
Mg2+ enhances activity at 1 mM or 10 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ Mycobacterium tuberculosis the enzyme is involved in betaq-oxidation cycle 3-acetoacetyl-CoA + NADH + H+
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ Mycobacterium tuberculosis ATCC 25618 the enzyme is involved in betaq-oxidation cycle 3-acetoacetyl-CoA + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNP7
-
-
Mycobacterium tuberculosis ATCC 25618 P9WNP7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxybutyryl-CoA + NAD+ the enzyme is involved in betaq-oxidation cycle Mycobacterium tuberculosis 3-acetoacetyl-CoA + NADH + H+
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ no activity woth NADP+ Mycobacterium tuberculosis 3-acetoacetyl-CoA + NADH + H+
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ the enzyme is involved in betaq-oxidation cycle Mycobacterium tuberculosis ATCC 25618 3-acetoacetyl-CoA + NADH + H+
-
r
(S)-3-hydroxybutyryl-CoA + NAD+ no activity woth NADP+ Mycobacterium tuberculosis ATCC 25618 3-acetoacetyl-CoA + NADH + H+
-
r
3-acetoacetyl-CoA + NADH + H+
-
Mycobacterium tuberculosis (S)-3-hydroxybutyryl-CoA + NAD+
-
r
3-acetoacetyl-CoA + NADH + H+
-
Mycobacterium tuberculosis ATCC 25618 (S)-3-hydroxybutyryl-CoA + NAD+
-
r
3-acetoacetyl-CoA + NADPH + H+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis (S)-3-hydroxybutyryl-CoA + NAD+
-
ir
3-acetoacetyl-CoA + NADPH + H+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis ATCC 25618 (S)-3-hydroxybutyryl-CoA + NAD+
-
ir

Synonyms

Synonyms Comment Organism
FadB2
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
NAD+ pH 9.5, 30°C Mycobacterium tuberculosis
0.011
-
3-acetoacetyl-CoA pH 7.0, 30°C Mycobacterium tuberculosis
0.012
-
(S)-3-hydroxybutyryl-CoA pH 9.5, 30°C Mycobacterium tuberculosis
0.189
-
NADH pH 7.0, 30°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA decreases above pH 6.5 Mycobacterium tuberculosis
10
-
NAD+-dependent dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
9 11 pH 9.0: about 45% of maximal activity, pH 11.0: about 55% of maximal activity Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+ no dehydration of beta-hydroxybutyryl-CoA to acetoacetyl-CoA with NADP+ as cofactor Mycobacterium tuberculosis
NADH conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA Mycobacterium tuberculosis
NADPH conversion of acetoacetyl-CoA to beta-hydroxybutyryl-CoA Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis calculated, native protein
-
5.2
Mycobacterium tuberculosis calculated, recombinant protein
-
6.1

General Information

General Information Comment Organism
malfunction a null mutant of fadB2 shows no significant differences from the wild-type strain with regard to lipid composition, utilization of different fatty acid carbon sources and tolerance to various stresses Mycobacterium tuberculosis
metabolism the enzyme is involved in beta-oxidation cycle Mycobacterium tuberculosis