Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Medicago sativa |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0), 20% (w/v) polyethylene glycol 6000, 0.1 M MgCl2, at 4°C | Medicago sativa |
Protein Variants | Comment | Organism |
---|---|---|
Y164A | the mutation abolishes enzymatic activity | Medicago sativa |
Y164G | the mutation abolishes enzymatic activity | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-vestitone + NADPH + H+ | Medicago sativa | - |
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-vestitone + NADPH + H+ | - |
Medicago sativa | (3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Medicago sativa |