Application | Comment | Organism |
---|---|---|
synthesis | 2,5-DKG reductase is an integral part of several industrial processes designed to synthesize 2-dehydro-L-gulonate based on the 2,5-diketo-D-gluconic acid pathway | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
gene dkr, recombinant expression in Lactococcus lactis strain NZ3900, and Lactobacillus plantarum strains WCFS1 and TLG02, cloning in Escherichia coli strains MB2159 and NEB 5-alpha, expression method evaluation | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishment of an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. Food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) are evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum, overview. Lactobacillus plantarum/pSIP609 is an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production. Highest production levels of 2,5-DKG reductase are obtained with the system Lactobacillus plantarum/pSIP609, resulting in 104 U/l without pH regulation and 262 U/l with pH control at pH 6.5 | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-L-gulonate + NADP+ | Corynebacterium glutamicum | - |
2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
2-dehydro-L-gulonate + NADP+ | Corynebacterium glutamicum DSM 20301 | - |
2,5-didehydro-D-gluconate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Corynebacterium glutamicum DSM 20301 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.188 | - |
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
0.191 | - |
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
0.232 | - |
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
0.243 | - |
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in not-pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
0.264 | - |
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
0.308 | - |
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in pH-regulated cultivation, pH 6.5, 22°C | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-L-gulonate + NADP+ | - |
Corynebacterium glutamicum | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
? | |
2-dehydro-L-gulonate + NADP+ | - |
Corynebacterium glutamicum DSM 20301 | 2,5-didehydro-D-gluconate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, recombinant enzyme, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconic acid reductase | - |
Corynebacterium glutamicum |
2,5-DKG reductase | - |
Corynebacterium glutamicum |
Dkr | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Corynebacterium glutamicum | |
NADPH | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | 2,5-DKG reductase catalyses the stereospecific reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) at position C-5 to 2-keto-L-gulonic acid (2-KLG), a key intermediate in the production of L-ascorbic acid | Corynebacterium glutamicum |
physiological function | the enzyme catalyses the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid | Corynebacterium glutamicum |