BRENDA - Enzyme Database
show all sequences of 1.1.1.346

Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Kaswurm, V.; Nguyen, T.T.; Maischberger, T.; Kulbe, K.D.; Michlmayr, H.; AMB Express 3, 7-17 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
2,5-DKG reductase is an integral part of several industrial processes designed to synthesize 2-dehydro-L-gulonate based on the 2,5-diketo-D-gluconic acid pathway
Corynebacterium glutamicum
Cloned(Commentary)
Cloned (Commentary)
Organism
gene dkr, recombinant expression in Lactococcus lactis strain NZ3900, and Lactobacillus plantarum strains WCFS1 and TLG02, cloning in Escherichia coli strains MB2159 and NEB 5-alpha, expression method evaluation
Corynebacterium glutamicum
Engineering
Protein Variants
Commentary
Organism
additional information
establishment of an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. Food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) are evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum, overview. Lactobacillus plantarum/pSIP609 is an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production. Highest production levels of 2,5-DKG reductase are obtained with the system Lactobacillus plantarum/pSIP609, resulting in 104 U/l without pH regulation and 262 U/l with pH control at pH 6.5
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-L-gulonate + NADP+
Corynebacterium glutamicum
-
2,5-didehydro-D-gluconate + NADPH + H+
-
-
?
2-dehydro-L-gulonate + NADP+
Corynebacterium glutamicum DSM 20301
-
2,5-didehydro-D-gluconate + NADPH + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum DSM 20301
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.188
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.191
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.232
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.243
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.264
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.308
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-dehydro-L-gulonate + NADP+
-
741575
Corynebacterium glutamicum
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
?
2-dehydro-L-gulonate + NADP+
-
741575
Corynebacterium glutamicum DSM 20301
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 31000, recombinant enzyme, SDS-PAGE
Corynebacterium glutamicum
Synonyms
Synonyms
Commentary
Organism
2,5-diketo-D-gluconic acid reductase
-
Corynebacterium glutamicum
2,5-DKG reductase
-
Corynebacterium glutamicum
Dkr
-
Corynebacterium glutamicum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Corynebacterium glutamicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Corynebacterium glutamicum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Corynebacterium glutamicum
NADPH
-
Corynebacterium glutamicum
Application (protein specific)
Application
Commentary
Organism
synthesis
2,5-DKG reductase is an integral part of several industrial processes designed to synthesize 2-dehydro-L-gulonate based on the 2,5-diketo-D-gluconic acid pathway
Corynebacterium glutamicum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene dkr, recombinant expression in Lactococcus lactis strain NZ3900, and Lactobacillus plantarum strains WCFS1 and TLG02, cloning in Escherichia coli strains MB2159 and NEB 5-alpha, expression method evaluation
Corynebacterium glutamicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Corynebacterium glutamicum
NADPH
-
Corynebacterium glutamicum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
establishment of an efficient process for 2,5-DKG reductase production that also satisfies food safety requirements. Food grade variants of the Lactobacillales based expression systems pSIP (Lactobacillus plantarum) and NICE (Lactococcus lactis) are evaluated with regard to their effictiveness to produce 2,5-DKG reductase from Corynebacterium glutamicum, overview. Lactobacillus plantarum/pSIP609 is an interesting alternative to Escherichia coli expression systems for industrial 2,5-DKG reductase production. Highest production levels of 2,5-DKG reductase are obtained with the system Lactobacillus plantarum/pSIP609, resulting in 104 U/l without pH regulation and 262 U/l with pH control at pH 6.5
Corynebacterium glutamicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2-dehydro-L-gulonate + NADP+
Corynebacterium glutamicum
-
2,5-didehydro-D-gluconate + NADPH + H+
-
-
?
2-dehydro-L-gulonate + NADP+
Corynebacterium glutamicum DSM 20301
-
2,5-didehydro-D-gluconate + NADPH + H+
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.188
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.191
-
recombinant enzyme, expressed in Lactococcus lactis strain NZ3900 from plasmid pVK51dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.232
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.243
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in not-pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.264
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP603dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
0.308
-
recombinant enzyme, expressed in Lactobacillus plantarum strain TLG02 from plasmid pSIP609dkr induced in pH-regulated cultivation, pH 6.5, 22C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-dehydro-L-gulonate + NADP+
-
741575
Corynebacterium glutamicum
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
?
2-dehydro-L-gulonate + NADP+
-
741575
Corynebacterium glutamicum DSM 20301
2,5-didehydro-D-gluconate + NADPH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 31000, recombinant enzyme, SDS-PAGE
Corynebacterium glutamicum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Corynebacterium glutamicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Corynebacterium glutamicum
General Information
General Information
Commentary
Organism
metabolism
2,5-DKG reductase catalyses the stereospecific reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) at position C-5 to 2-keto-L-gulonic acid (2-KLG), a key intermediate in the production of L-ascorbic acid
Corynebacterium glutamicum
physiological function
the enzyme catalyses the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid
Corynebacterium glutamicum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
2,5-DKG reductase catalyses the stereospecific reduction of 2,5-diketo-D-gluconic acid (2,5-DKG) at position C-5 to 2-keto-L-gulonic acid (2-KLG), a key intermediate in the production of L-ascorbic acid
Corynebacterium glutamicum
physiological function
the enzyme catalyses the reduction of 2,5-diketo-D-gluconic acid to 2-keto-L-gulonic acid, a direct precursor (lactone) of L-ascorbic acid (vitamin C). This reaction is an essential step in the biocatalytic production of the food supplement vitamin C from D-glucose or D-gluconic acid
Corynebacterium glutamicum
Other publictions for EC 1.1.1.346
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742259
Chen
Cloning, expression and chara ...
Comamonas testosteroni, Comamonas testosteroni ATCC 11996
Chem. Biol. Interact.
234
229-235
2015
-
-
1
-
1
-
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4
-
2
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1
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4
1
3
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1
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1
1
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1
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4
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1
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4
1
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-
-
-
-
1
3
3
1
-
-
741575
Kaswurm
Evaluation of the food grade ...
Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20301
AMB Express
3
7-17
2013
-
1
1
-
1
-
-
-
-
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2
-
2
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6
-
2
1
3
1
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1
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2
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1
1
2
-
1
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2
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-
-
6
-
2
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
657308
Sanli
Structural alteration of cofac ...
Corynebacterium sp.
Protein Sci.
13
504-512
2004
-
-
-
1
1
-
-
-
-
-
-
-
-
1
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-
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1
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2
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1
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1
1
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440310
Banta
Alteration of the specificity ...
Corynebacterium sp.
Protein Eng.
15
131-140
2002
-
-
1
-
40
-
-
-
-
-
1
-
-
2
-
-
1
-
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-
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-
2
1
3
-
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1
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1
1
-
40
-
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1
-
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1
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-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440311
Banta
Optimizing an artificial metab ...
Corynebacterium sp.
Biochemistry
41
6226-6236
2002
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
5
-
-
-
1
-
-
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-
-
-
1
-
5
-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
440312
Habrych
Purification and identificatio ...
Escherichia coli, Escherichia coli BL21-(DE3)
Biotechnol. Prog.
18
257-261
2002
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
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1
-
-
-
1
1
5
1
3
-
-
-
1
-
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1
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
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1
-
-
-
-
1
-
-
1
1
5
1
-
-
-
1
-
-
-
1
-
1
1
-
-
-
440307
Khurana
Molecular modeling of substrat ...
Corynebacterium sp.
Proteins
39
68-75
2000
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
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1
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1
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2
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-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
440306
Yum
Identification of the yqhE and ...
Escherichia coli
Appl. Environ. Microbiol.
65
3341-3346
1999
-
-
-
-
-
-
-
-
-
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3
-
-
8
-
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1
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2
1
7
-
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1
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3
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1
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-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum, Brevibacterium ketosoreductum ATCC 21914
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
-
-
2
-
-
1
-
-
-
2
-
5
1
2
-
-
-
-
1
-
1
1
-
1
-
-
-
-
1
-
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2
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2
-
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-
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1
-
-
2
-
5
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
440305
Khurana
Crystal structure of 2,5-diket ...
Corynebacterium sp.
Proc. Natl. Acad. Sci. USA
95
6768-6773
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
3
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1
-
-
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1
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3
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1
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1
1
1
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1
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1
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-
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-
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-
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-
-
-
721898
Maremonti
-
Characterisation of 2,5-diketo ...
Corynebacterium sp.
Biotechnol. Lett.
18
845-850
1996
-
-
-
-
-
-
-
1
-
-
2
-
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1
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1
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2
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1
1
2
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1
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1
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1
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2
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1
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2
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1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
440303
Sonoyama
-
Purification and properties of ...
Corynebacterium sp., Corynebacterium sp. SHS 0007
J. Ferment. Technol.
65
311-317
1987
-
-
-
-
-
-
-
4
-
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2
-
-
2
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1
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5
1
2
1
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1
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2
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1
1
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2
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1
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4
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2
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1
-
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-
-
5
1
1
-
1
-
2
-
1
2
-
-
-
-
-
-
440304
Miller
Purification and characterizat ...
Corynebacterium sp., Corynebacterium sp. ATCC 31090
J. Biol. Chem.
262
9016-9020
1987
-
-
-
-
-
-
6
6
1
-
2
-
-
2
-
-
1
-
-
-
2
2
5
1
2
-
-
-
1
2
1
-
1
1
1
-
-
-
-
1
-
-
-
-
6
1
6
1
-
2
-
-
-
-
1
-
-
2
2
5
1
-
-
-
1
2
1
-
1
-
-
-
-
-
-