BRENDA - Enzyme Database show
show all sequences of 1.1.1.346

Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli

Yum, D.Y.; Lee, B.Y.; Pan, J.G.; Appl. Environ. Microbiol. 65, 3341-3346 (1999)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Escherichia coli
30000
-
gel filtration
Escherichia coli
31003
-
1 * 31003, calculated from amino acid sequence
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli
Q46857
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-didehydro-D-gluconate + NADPH + H+
NADPH is the preferred electron donor
440306
Escherichia coli
2-dehydro-L-gulonate + NADP+
-
-
-
?
additional information
2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH
440306
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE; 1 * 31003, calculated from amino acid sequence
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
1 * 29000, SDS-PAGE
Escherichia coli
30000
-
gel filtration
Escherichia coli
31003
-
1 * 31003, calculated from amino acid sequence
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,5-didehydro-D-gluconate + NADPH + H+
NADPH is the preferred electron donor
440306
Escherichia coli
2-dehydro-L-gulonate + NADP+
-
-
-
?
additional information
2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH
440306
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE; 1 * 31003, calculated from amino acid sequence
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Escherichia coli
Other publictions for EC 1.1.1.346
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742259
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4
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-
1
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-
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-
1
1
-
1
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4
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-
-
1
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-
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1
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-
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3
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1
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2
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2
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6
-
2
1
1
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1
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2
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1
1
2
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1
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2
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-
6
-
2
1
1
-
-
-
1
-
-
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-
2
2
-
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-
657308
Sanli
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Corynebacterium sp.
Protein Sci.
13
504-512
2004
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-
-
1
1
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1
-
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1
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1
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1
1
1
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-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440310
Banta
Alteration of the specificity ...
Corynebacterium sp.
Protein Eng.
15
131-140
2002
-
-
1
-
40
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
40
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440311
Banta
Optimizing an artificial metab ...
Corynebacterium sp.
Biochemistry
41
6226-6236
2002
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
440312
Habrych
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Escherichia coli, Escherichia coli BL21-(DE3)
Biotechnol. Prog.
18
257-261
2002
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
-
1
-
-
-
1
1
5
1
-
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1
-
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-
1
-
1
-
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-
-
1
-
-
-
-
-
-
1
-
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1
-
-
-
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1
-
-
1
1
5
1
-
-
-
1
-
-
-
1
-
1
1
-
-
-
440307
Khurana
Molecular modeling of substrat ...
Corynebacterium sp.
Proteins
39
68-75
2000
-
-
-
-
2
-
-
-
-
-
-
-
-
1
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1
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1
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1
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2
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1
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-
440306
Yum
Identification of the yqhE and ...
Escherichia coli
Appl. Environ. Microbiol.
65
3341-3346
1999
-
-
-
-
-
-
-
-
-
-
3
-
-
8
-
-
1
-
-
-
-
-
2
1
-
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-
-
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
3
-
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1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
286278
Yum
Purification and characterizat ...
Brevibacterium ketosoreductum, Brevibacterium ketosoreductum ATCC 21914
Biosci. Biotechnol. Biochem.
62
154-156
1998
-
-
-
-
-
-
-
2
-
-
2
-
-
2
-
-
1
-
-
-
2
-
5
1
-
-
-
-
1
-
1
1
-
1
-
-
-
-
1
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
2
-
5
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
440305
Khurana
Crystal structure of 2,5-diket ...
Corynebacterium sp.
Proc. Natl. Acad. Sci. USA
95
6768-6773
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
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1
-
-
-
-
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1
-
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1
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1
1
1
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1
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1
-
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-
-
-
-
-
-
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-
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-
721898
Maremonti
-
Characterisation of 2,5-diketo ...
Corynebacterium sp.
Biotechnol. Lett.
18
845-850
1996
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
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1
-
-
-
2
-
1
1
-
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1
-
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-
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1
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1
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2
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1
-
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2
-
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
-
440303
Sonoyama
-
Purification and properties of ...
Corynebacterium sp., Corynebacterium sp. SHS 0007
J. Ferment. Technol.
65
311-317
1987
-
-
-
-
-
-
-
4
-
-
2
-
-
2
-
-
1
-
-
-
-
-
5
1
1
-
1
-
2
-
1
1
-
2
-
-
-
-
1
-
-
-
-
-
-
4
-
-
2
-
-
-
-
1
-
-
-
-
5
1
1
-
1
-
2
-
1
2
-
-
-
-
-
-
440304
Miller
Purification and characterizat ...
Corynebacterium sp., Corynebacterium sp. ATCC 31090
J. Biol. Chem.
262
9016-9020
1987
-
-
-
-
-
-
6
6
1
-
2
-
-
2
-
-
1
-
-
-
2
2
5
1
-
-
-
1
2
1
-
1
1
1
-
-
-
-
1
-
-
-
-
6
1
6
1
-
2
-
-
-
-
1
-
-
2
2
5
1
-
-
-
1
2
1
-
1
-
-
-
-
-
-