KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
4-methyl-2-oxopentanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
0.15 | - |
3-methyl-2-oxobutanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
0.26 | - |
phenylglyoxylate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
0.4 | - |
2-oxopentanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
0.6 | - |
2-Oxohexanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
0.76 | - |
(R)-mandelate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
3.4 | - |
2-oxo-3-phenylpropanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis | |
4 | - |
2-oxobutanoate | pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
40000 | - |
x * 40000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
110000 | - |
gel filtration, D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
130000 | - |
gel filtration, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterococcus faecalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
3.7 | - |
pH 7.5, 30°C, D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelate + NAD+ | Vmax/Km is 0.4% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | phenylglyoxylate + NADH + H+ | - |
? | |
(R)-mandelate + NAD+ | Vmax/Km is 1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | phenylglyoxylate + NADH + H+ | - |
? | |
2-oxo-3-phenylpropanoate + NADH + H+ | Vmax/KM is 1.9% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
2-oxo-3-phenylpropanoate + NADH + H+ | Vmax/Km is 2.7% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
2-oxobutanoate + NADH + H+ | Vmax/Km is 0.25% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxybutanoate + NAD+ | - |
? | |
2-oxobutanoate + NADH + H+ | Vmax/KM is less than 1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxybutanoate + NAD+ | - |
? | |
2-oxohexanoate + NADH + H+ | Vmax/Km is 4.1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxyhexanoate + NAD+ | - |
? | |
2-oxohexanoate + NADH + H+ | Vmax/Km is 5.1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxyhexanoate + NAD+ | - |
? | |
2-oxopentanoate + NADH + H+ | Vmax/Km is 5.8% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxypentanoate + NAD+ | - |
? | |
2-oxopentanoate + NADH + H+ | Vmax/Km is 7.6% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxypentanoate + NAD+ | - |
? | |
3-methyl-2-oxobutanoate + NADH + H+ | Vmax/Km is 51% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 3-methyl-2-hydroxybutanoate + NAD+ | - |
? | |
3-methyl-2-oxobutanoate + NADH + H+ | Vmax/Km is 85.7% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 3-methyl-2-hydroxybutanoate + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NADH + H+ | highest Vmax/Km value of all substrates tested | Enterococcus faecalis | 4-methyl-2-hydroxypentanoate + NAD+ | - |
? | |
additional information | both enzymes (D-mandelate dehydrogenase D-ManDH1 and D-mandelate dehydrogenase D-ManDH2) exhibit no or very little activity toward small 2-ketoacid substrates, such as pyruvate, hydroxypyruvate, and 2-ketobutyrate, and much higher activity toward substrates with larger aliphatic or aromatic side chains. The two enzymes exhibit higher activity (smaller Km and larger Vmax) for 2-ketoacid substrates branched at the C3 or C4 position than for unbranched substrates; i.e., 2-ketoisovalerate and 2-ketoisocaproate are more favorable than 2-ketovalerate and 2-ketocaproate, respectively. Among aromatic substrates, the two enzymes preferr benzoylformate to phenylpyruvate by 9- and 17-fold, respectively | Enterococcus faecalis | ? | - |
? | |
additional information | both enzymes (D-mandelate dehydrogenase D-ManDH1 and D-mandelate dehydrogenase D-ManDH2) exhibit no or very little activity toward small 2-oxoacid substrates, such as pyruvate, hydroxypyruvate, and 2-oxobutyrate, and much higher activity toward substrates with larger aliphatic or aromatic side chains. The two enzymes exhibit higher activity (smaller Km and larger Vmax) for 2-ketoacid substrates branched at the C3 or C4 position than for unbranched substrates; i.e., 2-oxoisovalerate and 2-oxoisocaproate are more favorable than 2-oxovalerate and 2-oxocaproate, respectively. Among aromatic substrates, the two enzymes prefer benzoylformate to phenylpyruvate by 9- and 17-fold, respectively | Enterococcus faecalis | ? | - |
? | |
phenylglyoxylate + NADH + H+ | Vmax/Km is 16.7% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | hydroxy(phenyl)acetic acid + NAD+ | - |
? | |
phenylglyoxylate + NADH + H+ | Vmax/Km is 44% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | hydroxy(phenyl)acetic acid + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
? | x * 40000, SDS-PAGE, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
D-mandelate dehydrogenase | - |
Enterococcus faecalis |
D-mandelate dehydrogenase | misleading | Enterococcus faecalis |
D-ManDH1 | the strain contains at least two distinct forms of D-ManDH, the two apparent D-mandelate dehydrogenases are named D-ManDH1 and D-ManDH2 according to the order of their elution | Enterococcus faecalis |
D-ManDH2 | the strain contains at least two distinct forms of D-ManDH, the two apparent D-mandelate dehydrogenases are named D-ManDH1 and D-ManDH2 according to the order of their elution | Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Enterococcus faecalis |
30 | 40 | D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
35 | 45 | D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
maximal catalytic efficiency, D-mandelate dehydrogenase D-ManDH1 | Enterococcus faecalis |
4.5 | - |
maximal catalytic efficiency, D-mandelate dehydrogenase D-ManDH2 | Enterococcus faecalis |
7.5 | - |
assay at | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH can not be replaced by NADPH in the benzoylformate reduction by D-mandelate dehydrogenase D-ManDH1 or D-ManDH2 | Enterococcus faecalis |