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Literature summary for 1.1.1.345 extracted from

  • Taguchi, H.; Ohta, T.
    D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family: Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum (1991), J. Biol. Chem., 266, 12588-12594.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MV1184 cells Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
pyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
2.9
-
3-hydroxypyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
20
-
phenylpyruvate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
28
-
2-oxobutyrate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
40
-
2-oxocaproate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
46
-
2-oxoisovalerate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
62
-
2-oxovalerate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
71
-
2-oxoisocaproate in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Lactiplantibacillus plantarum
37183
-
2 * 37183, calculated from amino acid sequence Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum ATCC 8041
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose DE32 column chromatography, AMP-Sepharose column chromatography, and Mono Q column chromatography Lactiplantibacillus plantarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
crude extract, in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum
170
-
after 77fold purification, in 100 mM Tris-HC1 buffer, pH 8.0, at 30°C Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
2-oxobutyrate + NADH + H+
-
Lactiplantibacillus plantarum ATCC 8041 ?
-
?
2-oxocaproate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
2-oxocaproate + NADH + H+
-
Lactiplantibacillus plantarum ATCC 8041 ?
-
?
2-oxoisocaproate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
2-oxoisocaproate + NADH + H+
-
Lactiplantibacillus plantarum ATCC 8041 ?
-
?
2-oxoisovalerate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
2-oxoisovalerate + NADH + H+
-
Lactiplantibacillus plantarum ATCC 8041 ?
-
?
2-oxovalerate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
3-hydroxypyruvate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
phenylpyruvate + NADH + H+
-
Lactiplantibacillus plantarum ?
-
?
pyruvate + NADH + H+
-
Lactiplantibacillus plantarum lactate + NAD+
-
?
pyruvate + NADH + H+
-
Lactiplantibacillus plantarum ATCC 8041 lactate + NAD+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 37000, SDS-PAGE Lactiplantibacillus plantarum
homodimer 2 * 37183, calculated from amino acid sequence Lactiplantibacillus plantarum

Synonyms

Synonyms Comment Organism
D-isomer-specific 2-hydroxyacid dehydrogenase
-
Lactiplantibacillus plantarum
D-lactate dehydrogenase
-
Lactiplantibacillus plantarum
D-lactate:NAD+ oxidoreductase
-
Lactiplantibacillus plantarum