Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
4-methyl-2-oxopentanoate | pH 7.5, 30°C | Enterococcus faecalis | |
0.18 | - |
3-methyl-2-oxobutanoate | pH 7.5, 30°C | Enterococcus faecalis | |
0.25 | - |
phenylglyoxylate | pH 7.5, 30°C | Enterococcus faecalis | |
0.3 | - |
2-oxopentanoate | pH 7.5, 30°C | Enterococcus faecalis | |
0.9 | - |
(R)-mandelate | pH 7.5, 30°C | Enterococcus faecalis | |
1.5 | - |
2-Oxohexanoate | pH 7.5, 30°C | Enterococcus faecalis | |
5.7 | - |
2-oxo-3-phenylpropanoate | pH 7.5, 30°C | Enterococcus faecalis | |
9.5 | - |
2-oxobutanoate | pH 7.5, 30°C | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34331 | - |
x * 34331, calculated from nucleotide sequence | Enterococcus faecalis |
40000 | - |
x * 40000, SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelate + NAD+ | Vmax/KM is less than 1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | phenylglyoxylate + NADH + H+ | - |
? | |
2-oxo-3-phenylpropanoate + NADH + H+ | Vmax/KM is 1.9% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxy-3-phenylpropanoate + NAD+ | - |
? | |
2-oxobutanoate + NADH + H+ | Vmax/KM is less than 1% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxybutanoate + NAD+ | - |
? | |
2-oxohexanoate + NADH + H+ | Vmax/KM is 3% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxyhexanoate + NAD+ | - |
? | |
2-oxopentanoate + NADH + H+ | Vmax/KM is 10.6% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 2-hydroxypentanoate + NAD+ | - |
? | |
3-methyl-2-oxobutanoate + NADH + H+ | Vmax/KM is 56% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | 3-methyl-2-hydroxybutanoate + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NADH + H+ | highest Vmax/Km value of all substrates tested | Enterococcus faecalis | 4-methyl-2-hydroxypentanoate + NAD+ | - |
? | |
additional information | the recombinant enzyme exhibits high catalytic activity toward various 2-oxoacid substrates with bulky hydrophobic side chains, particularly C3-branched substrates such as benzoylformate and 2-oxoisovalerate | Enterococcus faecalis | ? | - |
? | |
phenylglyoxylate + NADH + H+ | Vmax/KM is 54% compared to 4-methyl-2-oxopentanoate | Enterococcus faecalis | hydroxy(phenyl)acetic acid + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Enterococcus faecalis |
? | x * 34331, calculated from nucleotide sequence | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
D-mandelate dehydrogenase | misleading | Enterococcus faecalis |
D-ManDH2 | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strict coenzyme specificity for NADH and NAD+ | Enterococcus faecalis | |
NADH | strict coenzyme specificity for NADH and NAD+ | Enterococcus faecalis |