KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | with 2-oxobutyrate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
0.086 | - |
NADH | with 2-oxoisocaproate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
0.096 | - |
NADH | with pyruvate as cosubstrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
0.14 | - |
D-lactate | in 0.1 M acetate buffer, pH 5.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
0.36 | - |
2-oxobutyrate | in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
0.56 | - |
pyruvate | in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei | |
2.3 | - |
2-oxoisocaproate | in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | maximum activity in the presence of 4 M NaCl | Haloferax mediterranei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59500 | - |
2 * 59500, CTAB-PAGE | Haloferax mediterranei |
62900 | - |
2 * 62900, SDS-PAGE | Haloferax mediterranei |
101400 | - |
gel filtration | Haloferax mediterranei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Haloferax mediterranei R4, ATCC 33500 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose column chromatography, DEAE-cellulose column chromatography, Q-Sepharose column chromatography, Sephacryl S-300 gel filtration, and Sephadex G25 gel filtration | Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.427 | - |
with 2-oxoisocaproate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei |
0.799 | - |
with 2-oxobutyrate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei |
1.462 | - |
with pyruvate as substrate, in 0.1 M glycine-NaOH buffer, pH 9.0, 4 M NaCl, at 52°C | Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH + H+ | 55% activity compared to pyruvate | Haloferax mediterranei | D-2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | 55% activity compared to pyruvate | Haloferax mediterranei R4, ATCC 33500 | D-2-hydroxybutyrate + NAD+ | - |
r | |
2-oxoisocaproate + NADH + H+ | 29% activity compared to pyruvate | Haloferax mediterranei | L-2-hydroxyisocaproate + NAD+ | - |
r | |
2-oxoisocaproate + NADH + H+ | 29% activity compared to pyruvate | Haloferax mediterranei R4, ATCC 33500 | L-2-hydroxyisocaproate + NAD+ | - |
r | |
D-2-hydroxybutyrate + NAD+ | - |
Haloferax mediterranei | 2-oxobutyrate + NADH + H+ | - |
r | |
D-2-hydroxybutyrate + NAD+ | - |
Haloferax mediterranei R4, ATCC 33500 | 2-oxobutyrate + NADH + H+ | - |
r | |
D-lactate + NAD+ | - |
Haloferax mediterranei | pyruvate + NADH + H+ | - |
r | |
L-2-hydroxycaproate + NAD+ | - |
Haloferax mediterranei | 2-oxocaproate + NADH + H+ | - |
r | |
additional information | no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate | Haloferax mediterranei | ? | - |
? | |
additional information | no significant activity with L-lactate, L-2-hydroxybutyrate, L-hydroxyisocaproate, phenylpyruvate, 2-oxomethyl-n-valerate, and 2-oxoisovalerate | Haloferax mediterranei R4, ATCC 33500 | ? | - |
? | |
pyruvate + NADH + H+ | 100% activity | Haloferax mediterranei | D-lactate + NAD+ | - |
r | |
pyruvate + NADH + H+ | 100% activity | Haloferax mediterranei R4, ATCC 33500 | D-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59500, CTAB-PAGE | Haloferax mediterranei |
homodimer | 2 * 62900, SDS-PAGE | Haloferax mediterranei |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent D-2-hydroxyacid dehydrogenase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
52 | - |
- |
Haloferax mediterranei |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | above 60°C there is a pronounced activity decrease, while there is nearly no activity at 75°C | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
optimum pH for reaction with pyruvate or 2-oxobutyrate | Haloferax mediterranei |
7.5 | 8.5 | optimum pH values for reaction with 2-oxoisocaproate | Haloferax mediterranei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Haloferax mediterranei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is strictly NAD+-dependent | Haloferax mediterranei |