Protein Variants | Comment | Organism |
---|---|---|
S577A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lovastatin | - |
Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | Arabidopsis thaliana | - |
(R)-mevalonate + CoA + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P14891 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | isoform HMGR1S is inactivated through phosphorylation at Ser577 by the AKIN10-GRIK1 kinase cascade system in vitro | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
anti-FLAG-tag magnetic bead chromatography | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01333 | - |
mutant enzyme S577A from transgenic line 2, at 30°C, pH not specified in the publication | Arabidopsis thaliana |
0.01644 | - |
mutant enzyme S577A from transgenic line 1, at 30°C, pH not specified in the publication | Arabidopsis thaliana |
0.01949 | - |
wild type enzyme from transgenic line 1, at 30°C, pH not specified in the publication | Arabidopsis thaliana |
0.03568 | - |
wild type enzyme from transgenic line 2, at 30°C, not pH not specified in the publication | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | - |
Arabidopsis thaliana | (R)-mevalonate + CoA + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl-CoA reductase 1 | - |
Arabidopsis thaliana |
HMG-CoA reductase | - |
Arabidopsis thaliana |
HMGR1S | - |
Arabidopsis thaliana |