Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.34 extracted from

  • Robertlee, J.; Kobayashi, K.; Tang, J.; Suzuki, M.; Muranaka, T.
    Evidence that the Arabidopsis thaliana 3-hydroxy-3-methylglutaryl-CoA reductase 1 is phosphorylated at Ser577 in planta (2018), Plant Biotechnol., 35, 1-7 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
S577A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
lovastatin
-
Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM used in assay conditions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Arabidopsis thaliana
-
(R)-mevalonate + CoA + 2 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P14891
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein isoform HMGR1S is inactivated through phosphorylation at Ser577 by the AKIN10-GRIK1 kinase cascade system in vitro Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
anti-FLAG-tag magnetic bead chromatography Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01333
-
mutant enzyme S577A from transgenic line 2, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.01644
-
mutant enzyme S577A from transgenic line 1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.01949
-
wild type enzyme from transgenic line 1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.03568
-
wild type enzyme from transgenic line 2, at 30°C, not pH not specified in the publication Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
Arabidopsis thaliana (R)-mevalonate + CoA + 2 NADP+
-
?

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl-CoA reductase 1
-
Arabidopsis thaliana
HMG-CoA reductase
-
Arabidopsis thaliana
HMGR1S
-
Arabidopsis thaliana