Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.34 extracted from

  • Kalita, R.; Patar, L.; Shasany, A.K.; Modi, M.K.; Sen, P.
    Molecular cloning, characterization and expression analysis of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Centella asiatica L. (2015), Mol. Biol. Rep., 42, 1431-1439.
    View publication on PubMed
Search for more literature for 1.1.1.34

Cloned(Commentary)

Cloned (Comment) Organism
gene HMGR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha Centella asiatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, about, sequence calculation Centella asiatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-mevalonate + CoA + 2 NADP+ Centella asiatica
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 r

Organism

Organism UniProt Comment Textmining
Centella asiatica W6APW1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Centella asiatica
-
additional information the enzyme shows a strong expression in nodes followed by leaves and stem. Roots record the lowest expression level among all the tissues tested Centella asiatica
-
node
-
 Centella asiatica
-
root
-
 Centella asiatica
-
stem
-
 Centella asiatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mevalonate + CoA + 2 NADP+
-
 Centella asiatica (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 r

Subunits

Subunits Comment Organism
? x * 58000, about, sequence calculation Centella asiatica

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Centella asiatica
HMGR
-
 Centella asiatica

pI Value

Organism Comment pI Value Maximum pI Value
Centella asiatica sequence calculation
-
 8.65

General Information

General Information Comment Organism
additional information structure-function analysis of HMGR, homology modelling using human HMGR, PDB ID 1DQ8A, as template, overview Centella asiatica
physiological function the enzyme plays an important role in catalyzing the first committed step of isoprenoid biosynthesis in the mevalonic acid pathway catalyzing the conversion of HMG-CoA to mevalonic acid in plants Centella asiatica