Literature summary for 1.1.1.325 extracted from

Supangat, S.; Choi, Y.K.; Park, Y.S.; Son, D.; Han, C.D.; Lee, K.H.
Expression, purification, crystallization and preliminary X-ray analysis of sepiapterin reductase from Chlorobium tepidum (2005), Acta Crystallogr. Sect. F, 61, 202-204.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and selenomethionine-substituted enzymes in Escherichia coli strain BL21(DE3)	Chlorobaculum tepidum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged wild-type and selenomethionine-substituted enzymes, hanging drop vapour diffusion method at 18°C, mixing of 0.004 ml of protein solution containing 10 mg/ml in 20 mM Tris-HCl, pH 8.0, by ultrafiltration, with 0.001 ml of reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 34% PEG 400, and 0.2 M guanidine hydrochloride, screening and method optimization, crystals of the wild-type enzyme are soaked in reservoir solution containing 1 mM NADP+ and 1 mM sepiapterin or containing 1 mM NADP+ and 1 mM N-acetylserotonin, respectively, X-ray diffraction structure determination and analysis at 2.1 A resolution	Chlorobaculum tepidum

Crystallization (Comment)

Organism

purified recombinant His-tagged wild-type and selenomethionine-substituted enzymes, hanging drop vapour diffusion method at 18°C, mixing of 0.004 ml of protein solution containing 10 mg/ml in 20 mM Tris-HCl, pH 8.0, by ultrafiltration, with 0.001 ml of reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 34% PEG 400, and 0.2 M guanidine hydrochloride, screening and method optimization, crystals of the wild-type enzyme are soaked in reservoir solution containing 1 mM NADP+ and 1 mM sepiapterin or containing 1 mM NADP+ and 1 mM N-acetylserotonin, respectively, X-ray diffraction structure determination and analysis at 2.1 A resolution

Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6-pyruvoyl-5,6,7,8-tetrahydropterin + 3 NADPH + 3 H+	Chlorobaculum tepidum	-	L-threo-7,8-dihydrobiopterin + 3 NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlorobaculum tepidum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and selenomethionine-substituted enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration	Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-pyruvoyl-5,6,7,8-tetrahydropterin + 3 NADPH + 3 H+	-	Chlorobaculum tepidum	L-threo-7,8-dihydrobiopterin + 3 NADP+	-	?

Synonyms

Synonyms	Comment	Organism
sepiapterin reductase	-	Chlorobaculum tepidum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	dependent on	Chlorobaculum tepidum