Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and selenomethionine-substituted enzymes in Escherichia coli strain BL21(DE3) | Chlorobaculum tepidum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged wild-type and selenomethionine-substituted enzymes, hanging drop vapour diffusion method at 18°C, mixing of 0.004 ml of protein solution containing 10 mg/ml in 20 mM Tris-HCl, pH 8.0, by ultrafiltration, with 0.001 ml of reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 34% PEG 400, and 0.2 M guanidine hydrochloride, screening and method optimization, crystals of the wild-type enzyme are soaked in reservoir solution containing 1 mM NADP+ and 1 mM sepiapterin or containing 1 mM NADP+ and 1 mM N-acetylserotonin, respectively, X-ray diffraction structure determination and analysis at 2.1 A resolution | Chlorobaculum tepidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl-5,6,7,8-tetrahydropterin + 3 NADPH + 3 H+ | Chlorobaculum tepidum | - |
L-threo-7,8-dihydrobiopterin + 3 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and selenomethionine-substituted enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl-5,6,7,8-tetrahydropterin + 3 NADPH + 3 H+ | - |
Chlorobaculum tepidum | L-threo-7,8-dihydrobiopterin + 3 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sepiapterin reductase | - |
Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Chlorobaculum tepidum |