Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.325 extracted from

  • Cho, S.H.; Na, J.U.; Youn, H.; Hwang, C.S.; Lee, C.H.; Kang, S.O.
    Sepiapterin reductase producing L-threo-dihydrobiopterin from Chlorobium tepidum (1999), Biochem. J., 340, 497-503.
No PubMed abstract available
Search for more literature for 1.1.1.325

General Stability

General Stability Organism
stability of the purified enzyme disappears in sodium citrate buffer and potassium phosphate buffer for 24 h at 4°C, it increases continuously in Tris-HCl buffer and in glycine/NaOH buffer Chlorobaculum tepidum

Inhibitors

Inhibitors Comment Organism Structure
additional information N-acetylserotonin and melatonin are no inhibitors Chlorobaculum tepidum
N-acetyldopamine
-
 Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 NADPH pH 8.8, 45°C Chlorobaculum tepidum
0.021
-
 sepiapterin pH 8.8, 45°C Chlorobaculum tepidum
4
-
 diacetyl pH 8.8, 45°C Chlorobaculum tepidum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Chlorobaculum tepidum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Chlorobaculum tepidum
55000
-
 gel filtration Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ Chlorobaculum tepidum
-
 L-threo-7,8-dihydrobiopterin + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+
-
 Chlorobaculum tepidum L-threo-7,8-dihydrobiopterin + NADP+
-
 ?
diacetyl + NADPH + H+
-
 Chlorobaculum tepidum ? + NADP+
-
 ?
L-threo-7,8-dihydrobiopterin + NADP+
-
 Chlorobaculum tepidum sepiapterin + NADPH + H+
-
 r
additional information the enzyme also shows activity with D-threo-dihydrobiopterin, L-threo-dihydroneopterin, and dihydrotepidopterin, and low activity with L-erythro-dihydrobiopterin Chlorobaculum tepidum ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 26000, SDS-PAGE Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
sepiapterin reductase
-
 Chlorobaculum tepidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Chlorobaculum tepidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 sepiapterin pH 8.8, 45°C Chlorobaculum tepidum
5
-
 NADPH pH 8.8, 45°C Chlorobaculum tepidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Chlorobaculum tepidum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 N-acetyldopamine pH 8.8, 45°C Chlorobaculum tepidum

General Information

General Information Comment Organism
additional information alignment of amino acid sequences near the N-termini of sepiapterin reductases from various sources, overview Chlorobaculum tepidum