BRENDA - Enzyme Database show
show all sequences of 1.1.1.324

Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa)

Hallahan, D.; West, J.; Wallsgrove, R.; Smiley, D.; Dawson, G.; Pickett, J.; Hamilton, J.; Arch. Biochem. Biophys. 318, 105-112 (1995)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
plant (4aR,7S,7aS)-nepetalactone can be used for synthesis of (4aR,7S,7aS)-nepetalactol, an aphid pheromone, useful in aphid control
Nepeta racemosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
79% inhibition at 1.0 mM, 87% at 10 mM
Nepeta racemosa
4-chloromercuribenzoate
complete inhibition at 1 mM and above
Nepeta racemosa
EDTA
44% inhibition at 100 mM
Nepeta racemosa
iodoacetamide
5% inhibition at 1.0 mM, 22% at 10 mM
Nepeta racemosa
NEM
55% inhibition at 1.0 mM, complete inhibition at 10 mM
Nepeta racemosa
Triton X-100
potent inhibition at 0.01-0.1%
Nepeta racemosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
NADP+
with 8-hydroxynerol, pH 9.5, 25°C
Nepeta racemosa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Nepeta racemosa
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
the enzyme contains zinc ions
Nepeta racemosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000 + 1 x 42000, SDS-PAGE
Nepeta racemosa
63000
-
gel filtration
Nepeta racemosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6E)-8-hydroxygeraniol + 2 NADP+
Nepeta racemosa
-
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
Nepeta racemosa
-
? + 2 NADPH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nepeta racemosa
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration
Nepeta racemosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nepeta racemosa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1417
-
purified enzyme, pH 9.5, 25°C
Nepeta racemosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6E)-8-hydroxygeraniol + 2 NADP+
-
717149
Nepeta racemosa
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxygeraniol + 2 NADP+
oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis
717149
Nepeta racemosa
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
-
717149
Nepeta racemosa
? + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis
717149
Nepeta racemosa
? + 2 NADPH + 2 H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 40000 + 1 x 42000, SDS-PAGE
Nepeta racemosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Nepeta racemosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Nepeta racemosa
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Nepeta racemosa
NADP+
dependent on
Nepeta racemosa
Application (protein specific)
Application
Commentary
Organism
additional information
plant (4aR,7S,7aS)-nepetalactone can be used for synthesis of (4aR,7S,7aS)-nepetalactol, an aphid pheromone, useful in aphid control
Nepeta racemosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NAD+
Nepeta racemosa
NADP+
dependent on
Nepeta racemosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
79% inhibition at 1.0 mM, 87% at 10 mM
Nepeta racemosa
4-chloromercuribenzoate
complete inhibition at 1 mM and above
Nepeta racemosa
EDTA
44% inhibition at 100 mM
Nepeta racemosa
iodoacetamide
5% inhibition at 1.0 mM, 22% at 10 mM
Nepeta racemosa
NEM
55% inhibition at 1.0 mM, complete inhibition at 10 mM
Nepeta racemosa
Triton X-100
potent inhibition at 0.01-0.1%
Nepeta racemosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
NADP+
with 8-hydroxynerol, pH 9.5, 25°C
Nepeta racemosa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Nepeta racemosa
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
the enzyme contains zinc ions
Nepeta racemosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
1 * 40000 + 1 x 42000, SDS-PAGE
Nepeta racemosa
63000
-
gel filtration
Nepeta racemosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(6E)-8-hydroxygeraniol + 2 NADP+
Nepeta racemosa
-
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
Nepeta racemosa
-
? + 2 NADPH + 2 H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration
Nepeta racemosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nepeta racemosa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1417
-
purified enzyme, pH 9.5, 25°C
Nepeta racemosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(6E)-8-hydroxygeraniol + 2 NADP+
-
717149
Nepeta racemosa
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxygeraniol + 2 NADP+
oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis
717149
Nepeta racemosa
(6E)-8-oxogeranial + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
-
717149
Nepeta racemosa
? + 2 NADPH + 2 H+
-
-
-
?
(6E)-8-hydroxynerol + 2 NADP+
oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis
717149
Nepeta racemosa
? + 2 NADPH + 2 H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 40000 + 1 x 42000, SDS-PAGE
Nepeta racemosa
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Nepeta racemosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Nepeta racemosa
General Information
General Information
Commentary
Organism
metabolism
the enzyme plays a role in the biosynthesis of iridoid monoterpenes, e.g. (4aR,7S,7aS)-nepetalactone
Nepeta racemosa
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme plays a role in the biosynthesis of iridoid monoterpenes, e.g. (4aR,7S,7aS)-nepetalactone
Nepeta racemosa
Other publictions for EC 1.1.1.324
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717149
Hallahan
Purification and characterizat ...
Nepeta racemosa
Arch. Biochem. Biophys.
318
105-112
1995
-
1
-
-
-
-
6
1
1
1
2
2
-
3
-
-
1
-
-
1
1
-
4
1
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
6
-
1
1
1
2
2
-
-
-
1
-
1
1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
717741
Ikeda
Acyclic monoterpene primary al ...
Rauvolfia serpentina
J. Biochem.
109
341-347
1991
-
-
-
-
-
1
3
1
-
2
2
2
-
2
-
-
1
-
-
-
1
1
6
1
1
-
-
-
2
-
-
2
1
1
-
-
-
-
2
-
-
1
-
3
1
1
-
2
2
2
-
-
-
1
-
-
1
1
6
1
1
-
-
-
2
-
-
1
-
1
1
-
-
-