BRENDA - Enzyme Database
show all sequences of 1.1.1.320

Isolation and expression of a Bacillus cereus gene encoding benzil reductase

Maruyama, R.; Nishizawa, M.; Itoi, Y.; Ito, S.; Inoue, M.; Biotechnol. Bioeng. 75, 630-633 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene yueD, Bacillus cereus library screening in Escherichia coli and cloning of the gene encoding the benzil reductase, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha
Bacillus cereus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-benzoin + NADP+
Bacillus cereus
stereospecific asymmetric reduction of benzil to (S)-benzoin
benzil + NADPH + H+
-
-
r
(S)-benzoin + NADP+
Bacillus cereus Tim-r01
stereospecific asymmetric reduction of benzil to (S)-benzoin
benzil + NADPH + H+
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Bacillus cereus
Q8RJB2
-
-
Bacillus cereus Tim-r01
Q8RJB2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-benzoin + NADP+
stereospecific asymmetric reduction of benzil to (S)-benzoin
717406
Bacillus cereus
benzil + NADPH + H+
-
-
-
r
(S)-benzoin + NADP+
stereospecific asymmetric reduction of benzil to (S)-benzoin
717406
Bacillus cereus Tim-r01
benzil + NADPH + H+
-
-
-
r
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Bacillus cereus
NADPH
-
Bacillus cereus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene yueD, Bacillus cereus library screening in Escherichia coli and cloning of the gene encoding the benzil reductase, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Bacillus cereus
NADPH
-
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-benzoin + NADP+
Bacillus cereus
stereospecific asymmetric reduction of benzil to (S)-benzoin
benzil + NADPH + H+
-
-
r
(S)-benzoin + NADP+
Bacillus cereus Tim-r01
stereospecific asymmetric reduction of benzil to (S)-benzoin
benzil + NADPH + H+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-benzoin + NADP+
stereospecific asymmetric reduction of benzil to (S)-benzoin
717406
Bacillus cereus
benzil + NADPH + H+
-
-
-
r
(S)-benzoin + NADP+
stereospecific asymmetric reduction of benzil to (S)-benzoin
717406
Bacillus cereus Tim-r01
benzil + NADPH + H+
-
-
-
r
General Information
General Information
Commentary
Organism
evolution
the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, family
Bacillus cereus
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is a member of the short-chain dehydrogenase/reductase, SDR, family
Bacillus cereus
Other publictions for EC 1.1.1.320
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739919
Contente
Stereoselective reduction of a ...
Ogataea glucozyma
Appl. Microbiol. Biotechnol.
100
193-201
2016
-
-
-
-
-
-
-
1
-
-
-
-
-
3
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16
-
1
1
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1
1
1
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2
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1
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-
-
-
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16
-
1
-
1
1
1
-
2
-
-
-
-
-
1
1
717892
Maruyama
The enzymes with benzil reduct ...
Bacillus cereus
J. Biotechnol.
94
157-169
2002
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1
1
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2
-
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-
1
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6
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1
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-
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8
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1
1
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2
2
-
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2
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1
1
2
-
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2
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1
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1
-
-
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-
8
-
1
-
-
2
2
-
-
-
-
2
2
-
2
2
717406
Maruyama
Isolation and expression of a ...
Bacillus cereus, Bacillus cereus Tim-r01
Biotechnol. Bioeng.
75
630-633
2001
-
-
1
-
-
-
-
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2
-
7
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2
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2
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1
2
-
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2
-
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-
-
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-
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2
-
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-
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-
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-
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1
1
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