Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R,S)-mevaldate | 20 mM or higher | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADH | - |
Sus scrofa | |
0.09 | - |
NADPH | - |
Sus scrofa | |
4 | - |
mevaldate | - |
Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH | Sus scrofa | - |
(R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+ | - |
ir | |
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH | Sus scrofa | - |
(S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, calcium phosphate gel, DEAE-cellulose, bentonite precipitation | Sus scrofa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(R)-mevalonate + NAD+ = mevaldate + NADH + H+ | formation of mevalonic acid is strongly favoured | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.63 | - |
partially purified liver enzyme | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH | - |
Sus scrofa | (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+ | - |
ir | |
(R)-3-hydroxy-3-methyl-5-oxovaleric acid + NADPH | - |
Sus scrofa | (R)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NADP+ | - |
ir | |
(S)-3-hydroxy-3-methyl-5-oxovaleric acid + NADH | - |
Sus scrofa | (S)-3-hydroxy-3-methyl-5-hydroxyvaleric acid + NAD+ | - |
ir | |
mevaldate + NADH + H+ | - |
Sus scrofa | (R)-mevalonate + NAD+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.6 | 6.9 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Sus scrofa | |
NADPH | - |
Rattus norvegicus |