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Literature summary for 1.1.1.318 extracted from
- Functional characterization of the eugenol synthase gene (RcEGS1) in rose (2018), Plant Physiol. Biochem., 129, 21-26 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EGS1, cloning from petals of Rosa chinensis cv. Old Blush, DNA and amino acid sequence determination, recombinant overexpression of His-tagged enzyme in Escherichia coli from pTRV2 vector | Rosa chinensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | virus-induced gene silencing using Agrobacterium strain DHA105 containing TRV vectors, the amounts of scent compounds are analyzed by GC/MS in the RcEGS1-silenced plants | Rosa chinensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | Rosa hybrid cultivar | - |
eugenol + acetate + NADP+ | - |
r |  |
| coniferyl acetate + NADPH + H+ | Rosa chinensis | - |
eugenol + acetate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rosa chinensis | M1FGX5 | cv. Old Blush | - |
| Rosa hybrid cultivar | - |
cv. Yunxiang | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Rosa chinensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | mainly detected in stamens and petals at the flower opening stage | Rosa hybrid cultivar | - |
| flower | mainly detected in stamens and petals at the flower opening stage. RcEGS1 is principally localized in the upper and lower epidermal layers, which are the major sites of scent emission in roses | Rosa chinensis | - |
| petal | - |
Rosa hybrid cultivar | - |
| petal | enzyme RcEGS1 has its highest expression levels in the petals compared to other tissues. It has higher transcript levels at the developmental blooming stage and lower levels at budding and senescence stages | Rosa chinensis | - |
| stamen | - |
Rosa hybrid cultivar | - |
| stamen | - |
Rosa chinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | - |
Rosa hybrid cultivar | eugenol + acetate + NADP+ | - |
r | |
| coniferyl acetate + NADPH + H+ | - |
Rosa chinensis | eugenol + acetate + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RcEGS1 | - |
Rosa chinensis |
| RhEGS1 | - |
Rosa hybrid cultivar |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rosa hybrid cultivar | |
| NADP+ | - |
Rosa chinensis | |
| NADPH | - |
Rosa hybrid cultivar | |
| NADPH | - |
Rosa chinensis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | downregulation of RcEGS1 expression in flowers by virus-induced gene silencing leads to a reduction of the relative content of eugenol compared to wild-type flowers | Rosa chinensis |
| physiological function | enzyme is responsible for eugenol biosynthesis in roses | Rosa chinensis |
| physiological function | enzyme is responsible for eugenol biosynthesis in roses. Major volatile compounds from flower of Rosa x hybrida cv. Yunxiang at stage 4 are analyzed by SPME-GC/MS, the highest amount of 2-phenylethanol, followed by eugenol and methyleugenol, overview | Rosa hybrid cultivar |
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Release 2023.1 (January 2023)
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