Cloned (Comment) | Organism |
---|---|
gene DcEGS1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli | Daucus carota subsp. sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl acetate + NADPH + H+ | Daucus carota subsp. sativus | - |
eugenol + acetate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota subsp. sativus | A0A166G7A0 | orange genotype | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Daucus carota subsp. sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young and mature leaves | Daucus carota subsp. sativus | - |
additional information | quantitative RT-PCR enzyme expression analysis in different tissues. Distribution of aromatic volatiles in different parts of the carrot plants, overview | Daucus carota subsp. sativus | - |
root | - |
Daucus carota subsp. sativus | - |
seed | - |
Daucus carota subsp. sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl acetate + NADPH + H+ | - |
Daucus carota subsp. sativus | eugenol + acetate + NADP+ | - |
r | |
additional information | purified recombinant DcE(I)GS1 catalyzes the formation of eugenol (70% of total products, EC 1.1.1.318), as well as isoeugenol (30% of total products,, EC 1.1.1.319) from coniferyl acetate | Daucus carota subsp. sativus | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DcE(I)GS1 | - |
Daucus carota subsp. sativus |
DcEGS1 | - |
Daucus carota subsp. sativus |
eugenol/isoeugenol synthase | - |
Daucus carota subsp. sativus |
More | see also EC 1.1.1.319 | Daucus carota subsp. sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant His-tagged enzyme | Daucus carota subsp. sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His-tagged enzyme | Daucus carota subsp. sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | activity range | Daucus carota subsp. sativus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Daucus carota subsp. sativus | |
NADPH | - |
Daucus carota subsp. sativus |
General Information | Comment | Organism |
---|---|---|
additional information | distribution of aromatic volatiles in different parts of the carrot plants, overview | Daucus carota subsp. sativus |
physiological function | eugenol/isoeugenol synthase (DcE(I)GS1) is an NADPH-dependent enzyme that converts coniferyl acetate to eugenol. This enzyme exhibits dual product specificity and yields propenylphenol isoeugenol alongside allylphenol eugenol | Daucus carota subsp. sativus |