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Literature summary for 1.1.1.318 extracted from

  • Anand, A.; Jayaramaiah, R.H.; Beedkar, S.D.; Singh, P.A.; Joshi, R.S.; Mulani, F.A.; Dholakia, B.B.; Punekar, S.A.; Gade, W.N.; Thulasiram, H.V.; Giri, A.P.
    Comparative functional characterization of eugenol synthase from four different Ocimum species: Implications on eugenol accumulation (2016), Biochim. Biophys. Acta, 1864, 1539-1547.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure Ocimum gratissimum
homology modeling of structure Ocimum tenuiflorum
homology modeling of structure Ocimum kilimandscharicum
homology modeling of structure Ocimum basilicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.5
-
coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
0.55
-
4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.7
-
coniferyl acetate pH 6.5, 25°C Ocimum basilicum
1.17
-
coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum
1.25
-
4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
1.7
-
4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum

Organism

Organism UniProt Comment Textmining
Ocimum basilicum A0A1B2U6R8
-
-
Ocimum gratissimum A0A1B2U6S7
-
-
Ocimum kilimandscharicum A0A1B2U6T4
-
-
Ocimum tenuiflorum A0A1B2U6S6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum gratissimum
-
flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum tenuiflorum
-
flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum kilimandscharicum
-
flower higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum basilicum
-
leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum gratissimum
-
leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum tenuiflorum
-
leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum kilimandscharicum
-
leaf higher transcript levels especially in young leaves and inflorescence, levels are positively correlated with eugenol contents Ocimum basilicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaryl acetate + NADPH + H+
-
Ocimum gratissimum chavicol + acetate + NADP+
-
?
4-coumaryl acetate + NADPH + H+
-
Ocimum tenuiflorum chavicol + acetate + NADP+
-
?
4-coumaryl acetate + NADPH + H+
-
Ocimum kilimandscharicum chavicol + acetate + NADP+
-
?
coniferyl acetate + NADPH + H+
-
Ocimum gratissimum eugenol + acetate + NADP+
-
?
coniferyl acetate + NADPH + H+
-
Ocimum tenuiflorum eugenol + acetate + NADP+
-
?
coniferyl acetate + NADPH + H+
-
Ocimum kilimandscharicum eugenol + acetate + NADP+
-
?
coniferyl acetate + NADPH + H+
-
Ocimum basilicum eugenol + acetate + NADP+
-
?
additional information no substrate: 4-coumaryl acetate, cinnamyl acetate Ocimum basilicum ?
-
?
additional information no substrate: cinnamyl acetate Ocimum gratissimum ?
-
?
additional information no substrate: cinnamyl acetate Ocimum tenuiflorum ?
-
?
additional information no substrate: cinnamyl acetate Ocimum kilimandscharicum ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.003
-
coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.003
-
4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
0.004
-
coniferyl acetate pH 6.5, 25°C Ocimum basilicum
0.011
-
coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
0.011
-
4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.013
-
4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum
0.028
-
coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0027
-
4-coumaryl acetate pH 6.5, 25°C Ocimum gratissimum
0.003
-
coniferyl acetate pH 6.5, 25°C Ocimum tenuiflorum
0.006
-
coniferyl acetate pH 6.5, 25°C Ocimum basilicum
0.007
-
coniferyl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.019
-
4-coumaryl acetate pH 6.5, 25°C Ocimum kilimandscharicum
0.023
-
coniferyl acetate pH 6.5, 25°C Ocimum gratissimum
0.024
-
4-coumaryl acetate pH 6.5, 25°C Ocimum tenuiflorum