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Literature summary for 1.1.1.315 extracted from

  • Belyaeva, O.V.; Johnson, M.P.; Kedishvili, N.Y.
    Kinetic analysis of human enzyme RDH10 defines the characteristics of a physiologically relevant retinol dehydrogenase (2008), J. Biol. Chem., 283, 20299-20308.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cell Homo sapiens
RDH10 is expressed the enzyme in insect Sf9 cells using the Baculovirus expression system. Purification of RDH10-His6 from Sf9 cells using nickel affinity chromatography produces an inactive enzyme. Therefore, microsomal preparations of RDH10 are used for its kinetic characterization Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
11-cis-retinol substrate inhibition above 0.005 mM Homo sapiens
9-cis-retinol substrate inhibition above 0.001 mM Homo sapiens
all-trans-retinol substrate inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
all-trans-retinol
-
Homo sapiens
0.035
-
all-trans-retinol microsomal preparations of RDH10, cofactor: NAD+ Homo sapiens
0.04
-
9-cis-retinol microsomal preparations of RDH10 Homo sapiens
0.06
-
11-cis-retinol microsomal preparations of RDH10 Homo sapiens
0.1
-
NAD+
-
Homo sapiens
0.1
-
NAD+ microsomal preparations of RDH10 Homo sapiens
0.4
-
all-trans-retinal
-
Homo sapiens
0.4
-
all-trans-retinal microsomal preparations of RDH10 Homo sapiens
6
-
9-cis-retinol
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39600
-
x * 39600, SDS-PAGE and calculated Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoform Rdh10
-
Homo sapiens Q8IZV5
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of His-tagged enzyme using nickel affinity chromatography results in an inactive enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, some loss of activity after repeated freeze-thawing cycles Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-cis-retinol + NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens 11-cis-retinal + NADH + H+
-
r
11-cis-retinol + NADP+
-
Homo sapiens 11-cis-retinal + NADPH
-
?
9-cis-retinol + NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens 9-cis-retinal + NADH + H+
-
r
9-cis-retinol + NADP+
-
Homo sapiens 9-cis-retinal + NADPH
-
?
all-trans-retinal + NADPH + H+
-
Homo sapiens all-tans-retinol + NADP+
-
r
all-trans-retinol + NAD+ RDH10 is a more efficient retinol dehydrogenase than a retinaldehyde reductase. Microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens all-trans-retinal + NADH + H+
-
r
all-trans-retinol + NADPH
-
Homo sapiens all-trans-retinal + NADP+
-
r
additional information dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development Homo sapiens ?
-
?
additional information enzyme does not recognizes retinol bound to cellular retinol-binding protein type I as a substrate and functions exclusively in the oxidative reaction in cells Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 39600, SDS-PAGE and calculated Homo sapiens

Synonyms

Synonyms Comment Organism
RDH10
-
Homo sapiens
retinol dehydrogenase 10
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ isoform RDH10 is strictly NAD+-dependent Homo sapiens
NAD+ microsomal preparations of RDH10 are not active in presence of NADP+ Homo sapiens
NADH
-
Homo sapiens