Cloned (Comment) | Organism |
---|---|
expression in Sf9 cell | Homo sapiens |
RDH10 is expressed the enzyme in insect Sf9 cells using the Baculovirus expression system. Purification of RDH10-His6 from Sf9 cells using nickel affinity chromatography produces an inactive enzyme. Therefore, microsomal preparations of RDH10 are used for its kinetic characterization | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
11-cis-retinol | substrate inhibition above 0.005 mM | Homo sapiens | |
9-cis-retinol | substrate inhibition above 0.001 mM | Homo sapiens | |
all-trans-retinol | substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
all-trans-retinol | - |
Homo sapiens | |
0.035 | - |
all-trans-retinol | microsomal preparations of RDH10, cofactor: NAD+ | Homo sapiens | |
0.04 | - |
9-cis-retinol | microsomal preparations of RDH10 | Homo sapiens | |
0.06 | - |
11-cis-retinol | microsomal preparations of RDH10 | Homo sapiens | |
0.1 | - |
NAD+ | - |
Homo sapiens | |
0.1 | - |
NAD+ | microsomal preparations of RDH10 | Homo sapiens | |
0.4 | - |
all-trans-retinal | - |
Homo sapiens | |
0.4 | - |
all-trans-retinal | microsomal preparations of RDH10 | Homo sapiens | |
6 | - |
9-cis-retinol | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39600 | - |
x * 39600, SDS-PAGE and calculated | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform Rdh10 | - |
Homo sapiens | Q8IZV5 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of His-tagged enzyme using nickel affinity chromatography results in an inactive enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, some loss of activity after repeated freeze-thawing cycles | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-cis-retinol + NAD+ | microsomal preparations of RDH10 are not active in presence of NADP+ | Homo sapiens | 11-cis-retinal + NADH + H+ | - |
r | |
11-cis-retinol + NADP+ | - |
Homo sapiens | 11-cis-retinal + NADPH | - |
? | |
9-cis-retinol + NAD+ | microsomal preparations of RDH10 are not active in presence of NADP+ | Homo sapiens | 9-cis-retinal + NADH + H+ | - |
r | |
9-cis-retinol + NADP+ | - |
Homo sapiens | 9-cis-retinal + NADPH | - |
? | |
all-trans-retinal + NADPH + H+ | - |
Homo sapiens | all-tans-retinol + NADP+ | - |
r | |
all-trans-retinol + NAD+ | RDH10 is a more efficient retinol dehydrogenase than a retinaldehyde reductase. Microsomal preparations of RDH10 are not active in presence of NADP+ | Homo sapiens | all-trans-retinal + NADH + H+ | - |
r | |
all-trans-retinol + NADPH | - |
Homo sapiens | all-trans-retinal + NADP+ | - |
r | |
additional information | dual physiological role of isoform RDH10: in the biosynthesis of 11-cis-retinaldehyde for vision and in the biosynthesis of all-trans-retinoic acid for differentiation and development | Homo sapiens | ? | - |
? | |
additional information | enzyme does not recognizes retinol bound to cellular retinol-binding protein type I as a substrate and functions exclusively in the oxidative reaction in cells | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39600, SDS-PAGE and calculated | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
RDH10 | - |
Homo sapiens |
retinol dehydrogenase 10 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | isoform RDH10 is strictly NAD+-dependent | Homo sapiens | |
NAD+ | microsomal preparations of RDH10 are not active in presence of NADP+ | Homo sapiens | |
NADH | - |
Homo sapiens |