Cloned (Comment) | Organism |
---|---|
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase gene ligC expressed in Escherichia coli | Sphingomonas paucimobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 1 h at 0.1 mM inhibits 98% of enzyme activity | Sphingomonas paucimobilis | |
HgCl2 | 1 h at 0.01 mM inhibits 83% of enzyme activity | Sphingomonas paucimobilis | |
p-chloromercuribenzoate | 1 h at 0.01 mM inhibits 78% of enzyme activity | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0206 | - |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | in the presence of NAD+ | Sphingomonas paucimobilis | |
0.0246 | - |
NADP+ | - |
Sphingomonas paucimobilis | |
0.026 | - |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | in the presence of NADP+ | Sphingomonas paucimobilis | |
0.252 | - |
NAD+ | - |
Sphingomonas paucimobilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34590 | - |
1 * 34590, amino acid sequence | Sphingomonas paucimobilis |
67000 | - |
gel filtration | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
SYK6 | - |
Sphingomonas paucimobilis SYK6 | - |
SYK6 | - |
Purification (Comment) | Organism |
---|---|
from the cell extract of Escherichia coli, using POROS polyethyleneimine anion-exchange chromatography, POROS quaternized PI anion-exchange chromatography and POROS phenylether hydrophobic-interaction chromatography | Sphingomonas paucimobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
after purification from the cell extract of Escherichia coli | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ | - |
Sphingomonas paucimobilis | 4-carboxy-2-hydroxy-cis,cis-muconate + NADH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ | - |
Sphingomonas paucimobilis SYK6 | 4-carboxy-2-hydroxy-cis,cis-muconate + NADH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | - |
Sphingomonas paucimobilis | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid | ? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | - |
Sphingomonas paucimobilis SYK6 | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 34590, amino acid sequence | Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | can replace NADP+, but with lower affinity | Sphingomonas paucimobilis | |
NADP+ | - |
Sphingomonas paucimobilis |