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Literature summary for 1.1.1.312 extracted from

  • Maruyama, K.
    Interaction of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase with Reactive Blue 2 and related dyes (1988), J. Biochem., 103, 714-721.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Lubrol the enzyme activity increases in a sigmoidal manner with increasing concentrations, minimum concentration necessary for the increase of enzyme activity is higher than 0.05 mM, effectively eliminates the dye inhibition Pseudomonas straminea

Inhibitors

Inhibitors Comment Organism Structure
2-pyrone-4,6-dicarboxylic acid non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
Blue dextran competitive inhibitor with respect to NADP+ Pseudomonas straminea
methoxy Reactive Blue 2 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea
NADH competitive inhibition with respect to NAD+, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
NADPH competitive inhibition with respect to NADP, non-competitive inhibition with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde Pseudomonas straminea
Reactive blue 2 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea
Reactive Blue 4 inhibits the enzyme non-competitively with respect to 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and competitively with respect to NADP+ Pseudomonas straminea

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+ ordered bi-bi mechanism Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH following intramolecular dehydrogenation to the lactone 2-pyrone-4,6-dicarboxylic acid ?
additional information interaction with Blue Dextran-2000 and related dyes Pseudomonas straminea ?
-
?

Subunits

Subunits Comment Organism
dimer
-
Pseudomonas straminea

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Pseudomonas straminea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00006
-
Blue dextran
-
Pseudomonas straminea
0.0002
-
Reactive blue 2 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
0.0002
-
methoxy Reactive Blue 2 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
0.0045
-
Reactive Blue 4 coenzyme NADP+, similar value with coenzyme NAD+ Pseudomonas straminea
0.072
-
Reactive blue 2
-
Pseudomonas straminea