BRENDA - Enzyme Database show
show all sequences of 1.1.1.311

(S)-1-Phenylethanol dehydrogenase of Azoarcus sp. strain EbN1, an enzyme of anaerobic ethylbenzene catabolism

Kniemeyer, O.; Heider, J.; Arch. Microbiol. 176, 129-135 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-1-phenylethanol
oxidation of (S)-1-phenylethanol is completely inhibited in the presence of tenfold higher concentrations of (R)-1-phenylethanol
Azoarcus sp.
additional information
the presence of 10 mM EDTA (10 mM), 1 mM pyrazole or 1 mM 1,10-phenanthroline in enzyme assays does not affect the activity, indicating that the enzyme does not contain easily accessible bivalent metal ions. (S)-1-phenylethanol oxidation is not inhibited by 0.1 mM 5,5'-dithiobis-(2-nitrobenzoic acid), 1 mM 4-hydroxymercuribenzoic acid or 1 mM N-ethylmaleimide, indicating that no accessible cysteine residues are required for catalysis
Azoarcus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
acetophenone
pH 8, 30°C
Azoarcus sp.
0.0016
-
(S)-1-phenylethanol
pH 8, 30°C
Azoarcus sp.
0.0033
-
NADH
pH 8, 30°C
Azoarcus sp.
0.051
-
NAD+
pH 8, 30°C
Azoarcus sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25500
-
2 * 25500, SDS-PAGE
Azoarcus sp.
50000
-
gel filtration
Azoarcus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-1-phenylethanol + NAD+
Azoarcus sp.
-
acetophenone + NADH + H+
-
-
r
(S)-1-phenylethanol + NAD+
Azoarcus sp. EbN1
-
acetophenone + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Azoarcus sp.
-
-
-
Azoarcus sp. EbN1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Azoarcus sp.
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:ethylbenzene-grown cell
-
Azoarcus sp.
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
pH 8, 30°C
Azoarcus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-1-phenylethanol + NAD+
-
707292
Azoarcus sp.
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
no activity with NADP+. No activity with (R)-1-phenylethanol
707292
Azoarcus sp.
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
-
707292
Azoarcus sp. EbN1
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
no activity with NADP+. No activity with (R)-1-phenylethanol
707292
Azoarcus sp. EbN1
acetophenone + NADH + H+
-
-
-
r
additional information
no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol
707292
Azoarcus sp.
?
-
-
-
-
additional information
no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol
707292
Azoarcus sp. EbN1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 25500, SDS-PAGE
Azoarcus sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6
-
acetophenone
pH 8, 30°C
Azoarcus sp.
1.6
-
NADH
pH 8, 30°C
Azoarcus sp.
2
-
NAD+
pH 8, 30°C
Azoarcus sp.
2
-
(S)-1-phenylethanol
pH 8, 30°C
Azoarcus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
reduction of acetophenone
Azoarcus sp.
8
-
oxidation of (S)-1-phenylethanol
Azoarcus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Azoarcus sp.
NADH
-
Azoarcus sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Azoarcus sp.
two-dimensional gel electrophoresis
-
6.6
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Azoarcus sp.
NADH
-
Azoarcus sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-1-phenylethanol
oxidation of (S)-1-phenylethanol is completely inhibited in the presence of tenfold higher concentrations of (R)-1-phenylethanol
Azoarcus sp.
additional information
the presence of 10 mM EDTA (10 mM), 1 mM pyrazole or 1 mM 1,10-phenanthroline in enzyme assays does not affect the activity, indicating that the enzyme does not contain easily accessible bivalent metal ions. (S)-1-phenylethanol oxidation is not inhibited by 0.1 mM 5,5'-dithiobis-(2-nitrobenzoic acid), 1 mM 4-hydroxymercuribenzoic acid or 1 mM N-ethylmaleimide, indicating that no accessible cysteine residues are required for catalysis
Azoarcus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
acetophenone
pH 8, 30°C
Azoarcus sp.
0.0016
-
(S)-1-phenylethanol
pH 8, 30°C
Azoarcus sp.
0.0033
-
NADH
pH 8, 30°C
Azoarcus sp.
0.051
-
NAD+
pH 8, 30°C
Azoarcus sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25500
-
2 * 25500, SDS-PAGE
Azoarcus sp.
50000
-
gel filtration
Azoarcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-1-phenylethanol + NAD+
Azoarcus sp.
-
acetophenone + NADH + H+
-
-
r
(S)-1-phenylethanol + NAD+
Azoarcus sp. EbN1
-
acetophenone + NADH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Azoarcus sp.
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:ethylbenzene-grown cell
-
Azoarcus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
pH 8, 30°C
Azoarcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-1-phenylethanol + NAD+
-
707292
Azoarcus sp.
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
no activity with NADP+. No activity with (R)-1-phenylethanol
707292
Azoarcus sp.
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
-
707292
Azoarcus sp. EbN1
acetophenone + NADH + H+
-
-
-
r
(S)-1-phenylethanol + NAD+
no activity with NADP+. No activity with (R)-1-phenylethanol
707292
Azoarcus sp. EbN1
acetophenone + NADH + H+
-
-
-
r
additional information
no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol
707292
Azoarcus sp.
?
-
-
-
-
additional information
no activity with ethanol, 1-propanol, 1-butanol, 2-phenylethanol or benzyl alcohol
707292
Azoarcus sp. EbN1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 25500, SDS-PAGE
Azoarcus sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.6
-
acetophenone
pH 8, 30°C
Azoarcus sp.
1.6
-
NADH
pH 8, 30°C
Azoarcus sp.
2
-
NAD+
pH 8, 30°C
Azoarcus sp.
2
-
(S)-1-phenylethanol
pH 8, 30°C
Azoarcus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
reduction of acetophenone
Azoarcus sp.
8
-
oxidation of (S)-1-phenylethanol
Azoarcus sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Azoarcus sp.
two-dimensional gel electrophoresis
-
6.6
Other publictions for EC 1.1.1.311
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739947
Dudzik
Asymmetric reduction of ketone ...
Aromatoleum aromaticum, Aromatoleum aromaticum EbN1
Appl. Microbiol. Biotechnol.
99
5055-5069
2015
-
1
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685078
Hoeffken
Crystal structure and enzyme k ...
Azoarcus sp., Azoarcus sp. EbN1
Biochemistry
45
82-93
2006
-
-
1
1
-
-
1
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
707292
Kniemeyer
(S)-1-Phenylethanol dehydrogen ...
Azoarcus sp., Azoarcus sp. EbN1
Arch. Microbiol.
176
129-135
2001
-
-
-
-
-
-
2
4
-
-
2
2
-
11
-
-
1
-
-
1
1
-
6
1
-
-
-
4
2
-
-
2
-
1
-
-
-
-
2
-
-
-
-
2
-
4
-
-
2
2
-
-
-
1
-
1
1
-
6
1
-
-
-
4
2
-
-
1
-
-
-
-
-
-