Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | approximately 0.02-0.025 mM PhpC is incubated with 20-25 mM EDTA at 4°C until the activity is completely abolished (usually 1-2 h) | Streptomyces viridochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0193 | - |
NADH | in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication | Streptomyces viridochromogenes | |
0.185 | - |
phosphonoacetaldehyde | in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication | Streptomyces viridochromogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the iron content of PhpC is less than 5% | Streptomyces viridochromogenes | |
Zn2+ | Zn2+ is required for enzymatic activity | Streptomyces viridochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces viridochromogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces viridochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyethylphosphonate + NAD+ | the catalytic efficiency of phosphonoacetaldehyde reduction is 377fold greater than that of the reverse reaction, and even at pH 9.0, the catalytic efficiency of the forward reaction is still 11fold greater than that of the reverse reaction | Streptomyces viridochromogenes | phosphonoacetaldehyde + NADH + H+ | - |
r | |
additional information | no reaction is observed with hydroxymethylphosphonate and 3-hydroxypropylphosphonate | Streptomyces viridochromogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration | Streptomyces viridochromogenes |
Synonyms | Comment | Organism |
---|---|---|
PhpC | - |
Streptomyces viridochromogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
phosphonoacetaldehyde | in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication | Streptomyces viridochromogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
phosphonoacetaldehyde reduction is more favorable under neutral conditions (optimal pH at 7.0) | Streptomyces viridochromogenes |
9 | - |
2-hydroxyethylphosphonate oxidation is favored under basic conditions (optimal pH at 9.0) | Streptomyces viridochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | PhpC is an NADH-dependent enzyme | Streptomyces viridochromogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
phosphonoacetaldehyde | in 50 mM HEPES, 200 mM NaCl, pH 7.5, temperature not specified in the publication | Streptomyces viridochromogenes |