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Literature summary for 1.1.1.307 extracted from

  • Nidetzky, B.; Brueggler, K.; Kratzer, R.; Mayr, P.
    Multiple forms of xylose reductase in Candida intermedia comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies (2003), J. Agric. Food Chem., 51, 7930-7935 .
    View publication on PubMed
Search for more literature for 1.1.1.307

Inhibitors

Inhibitors Comment Organism Structure
NAD+
-
 [Candida] intermedia
NADH
-
 [Candida] intermedia
NADP+
-
 [Candida] intermedia
NADPH
-
 [Candida] intermedia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 NADP+ pH 7, 25°C [Candida] intermedia
0.37
-
 NAD+ pH 7, 25°C [Candida] intermedia
0.8
-
 xylitol cosubstrate NADP+, pH 7, 25°C [Candida] intermedia
0.9
-
 xylitol cosubstrate NAD+, pH 7, 25°C [Candida] intermedia
19
-
 NADH pH 7, 25°C [Candida] intermedia
30
-
 NADPH pH 7, 25°C [Candida] intermedia
30
-
 D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
64
-
 D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia

Organism

Organism UniProt Comment Textmining
[Candida] intermedia
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADH + H+
-
 [Candida] intermedia xylitol + NAD+
-
 r
D-xylose + NADPH + H+
-
 [Candida] intermedia xylitol + NADP+
-
 r
xylitol + NAD+
-
 [Candida] intermedia D-xylose + NADH + H+
-
 r
xylitol + NADP+
-
 [Candida] intermedia D-xylose + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
dsXR
-
 [Candida] intermedia
dual specific xylose reductase
-
 [Candida] intermedia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 NADH pH 7, 25°C [Candida] intermedia
16.3
-
 NADPH pH 7, 25°C [Candida] intermedia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 [Candida] intermedia
NADH
-
 [Candida] intermedia
NADP+
-
 [Candida] intermedia
NADPH dsXR binds NAD(P)+ weakly and NADH about as tightly as NADPH [Candida] intermedia

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0081
-
 NADH pH 7, 25°C [Candida] intermedia
0.014
-
 NADPH pH 7, 25°C [Candida] intermedia
0.053
-
 NADP+ pH 7, 25°C [Candida] intermedia
0.074
-
 NAD+ pH 7, 25°C [Candida] intermedia

General Information

General Information Comment Organism
metabolism ordered mechanism in which coenzyme binds first and substrate second [Candida] intermedia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
 xylitol cosubstrate NADP+, pH 7, 25°C [Candida] intermedia
0.023
-
 xylitol cosubstrate NAD+, pH 7, 25°C [Candida] intermedia
0.25
-
 D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia
0.53
-
 D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia