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Literature summary for 1.1.1.304 extracted from
- Cloning, expression, and characterization of budC gene encoding meso-2,3-butanediol dehydrogenase from Bacillus licheniformis (2016), Appl. Biochem. Biotechnol., 178, 604-617.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 0.5% of initial activity with substrate diacetyl, 0.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis |  |
| Al3+ | 1 mM, 4% of initial activity with substrate diacetyl, 6% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
| Cu2+ | 1 mM, 0.2% of initial activity with substrate diacetyl, 1% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
| EDTA | 1 mM, 91% of initial activity with substrate diacetyl, 90% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
| Fe3+ | 1 mM, 2% of initial activity with substrate diacetyl, 3.5% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
| Zn2+ | 1 mM, 75% of initial activity with substrate diacetyl, 80% with substrate 2,3-butanediol, respectively | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis | |
| 0.34 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
| 0.47 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
| 7.25 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
| 72.4 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Bacillus licheniformis |
| 125000 | - |
gel filtration | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 120 | - |
substrate diacetyl, 30°C, pH 6.0 | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S,3S)-2,3-butanediol + NAD+ | - |
Bacillus licheniformis | (3S)-acetoin + NADH + H+ | - |
r | |
| (3S)-acetoin + NADH + H+ | 97% of the activity with diacetyl | Bacillus licheniformis | (2S,3S)-2,3-butanediol + NAD+ | 97.3% enantiomeric excess and 96.5% diastereomeric excess | r | |
| 1,2-propanediol + NAD+ | - |
Bacillus licheniformis | ? + NADH + H+ | 0.5% of the activity with 2,3-butanediol | r | |
| 2,3-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 66% of the activity with diacetyl | ? | |
| 2,3-pentanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 69% of the activity with diacetyl | ? | |
| 3,4-hexanedione + NADH + H+ | - |
Bacillus licheniformis | ? + NAD+ | 10% of the activity with diacetyl | ? | |
| diacetyl + NADH + H+ | - |
Bacillus licheniformis | (3S)-acetoin + NAD+ | 97.3% enantiomeric excess | ir | |
| additional information | enzyme shows oxidative activity to racemic 2,3-butanediol but no activity toward racemic acetoin in the presence of NAD+ | Bacillus licheniformis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Bacillus licheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| budC | - |
Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 202 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
| 591 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
| 748 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
| 1222 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
| 1274 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
reduction of diacetyl | Bacillus licheniformis |
| 10 | - |
oxidation of butanediol | Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | reduction of diacetyl | Bacillus licheniformis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADPH | Bacillus licheniformis | |
| NAD+ | - |
Bacillus licheniformis | |
| NADH | - |
Bacillus licheniformis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.9 | - |
diacetyl | pH 6.0, 30°C | Bacillus licheniformis | |
| 81.5 | - |
(2S,3S)-2,3-butanediol | pH 10.0, 30°C | Bacillus licheniformis | |
| 432 | - |
(3S)-acetoin | pH 6.0, 30°C | Bacillus licheniformis | |
| 2192 | - |
NAD+ | pH 10.0, 30°C | Bacillus licheniformis | |
| 5072 | - |
NADH | pH 6.0, 30°C | Bacillus licheniformis | |
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