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Literature summary for 1.1.1.304 extracted from

  • Carballo, J.; Martin, R.; Bernardo, A.; Gonzalez, J.
    Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes (1991), Eur. J. Biochem., 198, 327-332.
    View publication on PubMed
Search for more literature for 1.1.1.304

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 NADH cosubstrate acetoin, pH 7.0, 25°C Klebsiella aerogenes
0.007
-
 NADH cosubstrate diacetyl, pH 7.0, 25°C Klebsiella aerogenes
1.6
-
 diacetyl pH 7.0, 25°C Klebsiella aerogenes
6
-
 pentane-2,3-dione pH 7.0, 25°C Klebsiella aerogenes
18
-
 Methyl pyruvate pH 7.0, 25°C Klebsiella aerogenes
20
-
 ethyl pyruvate pH 7.0, 25°C Klebsiella aerogenes
75
-
 methyl glyoxal pH 7.0, 25°C Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Klebsiella aerogenes
61000
-
 gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NADH + H+ 85.6% of the activity with diacetyl Klebsiella aerogenes 3-hydroxy-2-pentanone + NAD+
-
 ?
diacetyl + NADH + H+
-
 Klebsiella aerogenes (S)-acetoin + NAD+
-
 ?
ethyl pyruvate + NADH + H+ 57.7% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?
methyl glyoxal + NADH + H+ 11% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?
methyl pyruvate + NADH + H+ 49% of the activity with diacetyl Klebsiella aerogenes ? + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NADH specific for beta-NADH Klebsiella aerogenes

pI Value

Organism Comment pI Value Maximum pI Value
Klebsiella aerogenes isoelectric focusing
-
 6.8