Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation, (R)-2,3-butanediol dehydrogenase activity | Saccharomyces cerevisiae | |
Mn2+ | activation, (R)-2,3-butanediol dehydrogenase activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinate | inhibition, (R)-2,3-butanediol dehydrogenase activity | Saccharomyces cerevisiae | |
EDTA | inhibition, (R)-2,3-butanediol dehydrogenase activity | Saccharomyces cerevisiae | |
o-phenanthroline | inhibition, (R)-2,3-butanediol dehydrogenase activity | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
140000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-cyclohexanedione + NADH + H+ | 5% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin | Saccharomyces cerevisiae | (R)-2-hydroxy-1-cyclohexanone + NAD+ | - |
ir | |
2,3-pentanedione + NADH + H+ | 7% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin | Saccharomyces cerevisiae | (3R)-3-hydroxy-2-pentanone + NAD+ | - |
ir | |
diacetyl + NADH + H+ | 21% of the (R)-2,3-butanediol dehydrogenase activity with substrate acetoin | Saccharomyces cerevisiae | (R)-acetoin + NAD+ | - |
ir | |
additional information | enzyme is specific for NADH | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme is specific for NADH | Saccharomyces cerevisiae |