Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.30 extracted from

  • Yeon, Y.; Park, H.; Park, K.; Park, H.; Yoo, Y.
    Structural basis for the substrate specificity of 3-hydroxybutyrate dehydrogenase (2016), Biotechnol. Bioprocess Eng., 21, 364-372.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alcaligenes faecalis

Protein Variants

Protein Variants Comment Organism
H144A the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144F the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144I the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L/W187A the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L/W187F the mutant shows strongly increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L/W187I the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L/W187L the mutant shows strongly increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144L/W187V the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
H144V the mutant shows wild type catalytic efficiency with levulinic acid Alcaligenes faecalis
W187A the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
W187F the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
W187I the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
W187L the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis
W187V the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
levulinic acid mutant enzyme H144L/W187L, at pH 6.5 and 30°C Alcaligenes faecalis
0.63
-
levulinic acid mutant enzyme W187F, at pH 6.5 and 30°C Alcaligenes faecalis
0.64
-
levulinic acid mutant enzyme H144L/W187F, at pH 6.5 and 30°C Alcaligenes faecalis
0.78
-
levulinic acid wild type enzyme, at pH 6.5 and 30°C Alcaligenes faecalis
0.91
-
levulinic acid mutant enzyme H144L, at pH 6.5 and 30°C Alcaligenes faecalis
1.72
-
levulinic acid mutant enzyme H144F, at pH 6.5 and 30°C Alcaligenes faecalis
2.81
-
levulinic acid mutant enzyme H144I, at pH 6.5 and 30°C Alcaligenes faecalis
3.84
-
levulinic acid mutant enzyme H144V, at pH 6.5 and 30°C Alcaligenes faecalis
4 5.4 levulinic acid mutant enzyme W187I, at pH 6.5 and 30°C Alcaligenes faecalis
9.87
-
levulinic acid mutant enzyme H144A, at pH 6.5 and 30°C Alcaligenes faecalis
13.03
-
levulinic acid mutant enzyme H144L/W187A, at pH 6.5 and 30°C Alcaligenes faecalis
13.49
-
levulinic acid mutant enzyme W187L, at pH 6.5 and 30°C Alcaligenes faecalis
25.57
-
levulinic acid mutant enzyme H144L/W187V, at pH 6.5 and 30°C Alcaligenes faecalis
25.76
-
levulinic acid mutant enzyme H144L/W187I, at pH 6.5 and 30°C Alcaligenes faecalis
36.83
-
levulinic acid mutant enzyme W187A, at pH 6.5 and 30°C Alcaligenes faecalis
42.1
-
levulinic acid mutant enzyme W187V, at pH 6.5 and 30°C Alcaligenes faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetoacetate + NADH + H+ Alcaligenes faecalis
-
(R)-3-hydroxybutanoate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis D0VWQ0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetate + NADH + H+
-
Alcaligenes faecalis (R)-3-hydroxybutanoate + NAD+
-
?
levulinic acid + NADH + H+ the wild type enzyme shows low activity with levulinic acid, while it's a good substrate for mutant enzyme H144L/W187F Alcaligenes faecalis 4-hydroxyvaleric acid + NAD+
-
?

Synonyms

Synonyms Comment Organism
3HBDH
-
Alcaligenes faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00016
-
levulinic acid mutant enzyme W187A, at pH 6.5 and 30°C Alcaligenes faecalis
0.0003
-
levulinic acid mutant enzyme W187I, at pH 6.5 and 30°C Alcaligenes faecalis
0.0003
-
levulinic acid mutant enzyme W187V, at pH 6.5 and 30°C Alcaligenes faecalis
0.00084
-
levulinic acid mutant enzyme H144A, at pH 6.5 and 30°C Alcaligenes faecalis
0.00122
-
levulinic acid mutant enzyme H144L/W187A, at pH 6.5 and 30°C Alcaligenes faecalis
0.002
-
levulinic acid mutant enzyme H144L/W187V, at pH 6.5 and 30°C Alcaligenes faecalis
0.00289
-
levulinic acid mutant enzyme H144L/W187I, at pH 6.5 and 30°C Alcaligenes faecalis
0.00465
-
levulinic acid mutant enzyme W187L, at pH 6.5 and 30°C Alcaligenes faecalis
0.00766
-
levulinic acid mutant enzyme H144V, at pH 6.5 and 30°C Alcaligenes faecalis
0.00768
-
levulinic acid wild type enzyme, at pH 6.5 and 30°C Alcaligenes faecalis
0.00921
-
levulinic acid mutant enzyme H144F, at pH 6.5 and 30°C Alcaligenes faecalis
0.01069
-
levulinic acid mutant enzyme H144I, at pH 6.5 and 30°C Alcaligenes faecalis
0.02969
-
levulinic acid mutant enzyme W187F, at pH 6.5 and 30°C Alcaligenes faecalis
0.06594
-
levulinic acid mutant enzyme H144L, at pH 6.5 and 30°C Alcaligenes faecalis
0.07107
-
levulinic acid mutant enzyme H144L/W187L, at pH 6.5 and 30°C Alcaligenes faecalis
0.2565
-
levulinic acid mutant enzyme H144L/W187F, at pH 6.5 and 30°C Alcaligenes faecalis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Alcaligenes faecalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.006
-
levulinic acid wild type enzyme, at pH 6.5 and 30°C Alcaligenes faecalis
0.006
-
levulinic acid mutant enzyme W187A, at pH 6.5 and 30°C Alcaligenes faecalis
0.008
-
levulinic acid mutant enzyme H144A, at pH 6.5 and 30°C Alcaligenes faecalis
0.013
-
levulinic acid mutant enzyme W187V, at pH 6.5 and 30°C Alcaligenes faecalis
0.014
-
levulinic acid mutant enzyme W187I, at pH 6.5 and 30°C Alcaligenes faecalis
0.016
-
levulinic acid mutant enzyme H144F, at pH 6.5 and 30°C Alcaligenes faecalis
0.016
-
levulinic acid mutant enzyme H144L/W187A, at pH 6.5 and 30°C Alcaligenes faecalis
0.019
-
levulinic acid mutant enzyme W187F, at pH 6.5 and 30°C Alcaligenes faecalis
0.03
-
levulinic acid mutant enzyme H144I, at pH 6.5 and 30°C Alcaligenes faecalis
0.03
-
levulinic acid mutant enzyme H144V, at pH 6.5 and 30°C Alcaligenes faecalis
0.045
-
levulinic acid mutant enzyme H144L/W187L, at pH 6.5 and 30°C Alcaligenes faecalis
0.051
-
levulinic acid mutant enzyme H144L/W187V, at pH 6.5 and 30°C Alcaligenes faecalis
0.06
-
levulinic acid mutant enzyme H144L, at pH 6.5 and 30°C Alcaligenes faecalis
0.063
-
levulinic acid mutant enzyme W187L, at pH 6.5 and 30°C Alcaligenes faecalis
0.074
-
levulinic acid mutant enzyme H144L/W187I, at pH 6.5 and 30°C Alcaligenes faecalis
0.164
-
levulinic acid mutant enzyme H144L/W187F, at pH 6.5 and 30°C Alcaligenes faecalis