Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
H141A | kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 12.9fold lower than wild-type enzyme | Pseudomonas putida |
K149A | inactive mutant enzyme | Pseudomonas putida |
K149R | kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 7.2fold lower than wild-type enzyme | Pseudomonas putida |
Q193A | kcat/KM for (R)-3-hydroxybutanoate is 307fold lower than wild-type value, kcat/Km for NAD+ is 6.2fold lower than wild-type enzyme | Pseudomonas putida |
Q91A | kcat/KM for (R)-3-hydroxybutanoate is 83.7fold lower than wild-type value, kcat/Km for NAD+ is 2.8fold lower than wild-type enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NAD+ | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
0.27 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
0.28 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
0.38 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
0.51 | - |
NAD+ | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida | |
1.5 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
35 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
78 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
96 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
131 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q9AE70 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Pseudomonas putida | acetoacetate + NADH + H+ | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
167 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme H141A | Pseudomonas putida | |
333 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q193A | Pseudomonas putida | |
404 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme K149R | Pseudomonas putida | |
1382 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, wild-type enzyme | Pseudomonas putida | |
1390 | - |
(R)-3-hydroxybutanoate | 23°C, pH 8.0, mutant enzyme Q91A | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida |