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Literature summary for 1.1.1.30 extracted from
- Purification and characterization of the D-beta-hydroxybutyrate dehydrogenase from dromedary liver mitochondria (2002), Comp. Biochem. Physiol. B, 131, 9-18.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phospholipids | absolutely dependent on | Camelus dromedarius |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dissociation constants | Camelus dromedarius | |
| 0.21 | - |
NAD+ | pH 8.1, 37°C | Camelus dromedarius | |
| 0.27 | - |
NADH | pH 7.0, 37°C | Camelus dromedarius | |
| 0.29 | - |
acetoacetate | pH 7.0, 37°C | Camelus dromedarius | |
| 1.07 | - |
(R)-3-hydroxybutyrate | pH 8.1, 37°C | Camelus dromedarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Camelus dromedarius | 16020 | - |
| mitochondrion | - |
Camelus dromedarius | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, SDS-PAGE | Camelus dromedarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 1000fold, to homogeneity from liver mitochondrial membranes by solubilization with Triton X-100, DEAE-ion-exchange chromatography and phenyl-resin chromatography | Camelus dromedarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Camelus dromedarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.09 | - |
purified enzyme | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutyrate + NAD+ | - |
Camelus dromedarius | acetoacetate + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Camelus dromedarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BDH | - |
Camelus dromedarius |
| D-beta-hydroxybutyrate dehydrogenase | - |
Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Camelus dromedarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 30% of maximal activity at 20°C, 63% of maximal activity at 60°C | Camelus dromedarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
37 | stable at, 30% remaining activity after 40 min at 37°C, oxidation reaction | Camelus dromedarius |
| 45 | - |
25% remaining activity after 20 min, oxidation reaction | Camelus dromedarius |
| 60 | - |
28% remaining activity | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
approximately, reduction reaction | Camelus dromedarius |
| 8 | - |
approximately, oxidation reaction | Camelus dromedarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Camelus dromedarius | |
| NADH | - |
Camelus dromedarius | |
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