Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphatidylcholine | - |
Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
NAD+ | - |
Bacillus cereus | |
2.3 | - |
(R)-3-hydroxybutanoate | - |
Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.1 mM, 2fold increase in activity, crude extracts | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | Bacillus cereus | - |
acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | Bacillus cereus T | - |
acetoacetate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex, Sephadex G-200 | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21 | - |
- |
Bacillus cereus |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl buffer, 50-100 mM Mg2+, Mn2+, Ba2+, or Ca2+, enzyme loses all activity in the presence of phosphate buffer or EDTA within 2 min | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus | acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus T | acetoacetate + NADH | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus | acetoacetate + NADH + H+ | - |
r | |
(R)-3-hydroxybutanoate + NAD+ | - |
Bacillus cereus T | acetoacetate + NADH + H+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus |